Gene Trapping Using Gal4 in Zebrafish
by
Balciuniene, Jorune
Title
:
Gene Trapping Using Gal4 in Zebrafish
Author
:
Balciuniene, Jorune
Personal Author
:
Balciuniene, Jorune
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (814 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2256107-1001 | XX(2256107.1) | JoVe Video Online |