Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
by
Abrams, Jennifer L.
Title
:
Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
Author
:
Abrams, Jennifer L.
Personal Author
:
Abrams, Jennifer L.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (832 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
This article describes the use of a firefly luciferase-GFP fusion protein to investigate in vivo protein folding in Saccharomyces cerevisiae. Using this reagent, refolding of a model heat-denatured protein can be monitored simultaneously by fluorescence microscopy and an enzymatic assay to probe the roles of proteostasis network components in protein quality control.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2256268-1001 | XX(2256268.1) | JoVe Video Online |