Identification of Protein Interacting Partners Using Tandem Affinity Purification
by
Bailey, Dalan
Title
:
Identification of Protein Interacting Partners Using Tandem Affinity Purification
Author
:
Bailey, Dalan
Personal Author
:
Bailey, Dalan
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (602 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
Tandem affinity purification is a robust approach for the identification of protein binding partners. As proof of concept, this methodology was applied to the well-characterized translation initiation factor eIF4E to co-precipitate the host cell factors involved in translation initiation. This method is easily adapted to any cellular or viral protein.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2257121-1001 | XX(2257121.1) | JoVe Video Online |