Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
by
Aldred, Shelley Force
Title
:
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
Author
:
Aldred, Shelley Force
Personal Author
:
Aldred, Shelley Force
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (439 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
MicroRNAs (miRNAs) are important regulators of gene expression and have been shown to play a role in numerous biological processes. To better understand miRNA-UTR interactions, we have created a genome-wide collection of 3 UTR luciferase reporters paired with a novel luciferase gene and assay reagent, the LightSwitch system.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2257365-1001 | XX(2257365.1) | JoVe Video Online |