Identification of salt stress responsive protyeins in wild sugar beet (beta maritima) using 2D-page with maldi-tof/tof system
by
Çakıroğlu, Çiğdem.
Title
:
Identification of salt stress responsive protyeins in wild sugar beet (beta maritima) using 2D-page with maldi-tof/tof system
Author
:
Çakıroğlu, Çiğdem.
Personal Author
:
Çakıroğlu, Çiğdem.
Publication Information
:
[s.l.]: [s.n.], 2012.
Physical Description
:
xiii, 55 leaves. : ill. + 1 computer laser optical disc.
Abstract
:
High salinity is one of the abiotic stresses, which affects the homeostasis, growth and productivity of plants. In plants, uptake of the non-essential salt ions negatively affects the anatomy, physiology and metabolism, changes the osmotic balance in cells and causes abundant dehydration. In this case, higher plants develop salt tolerance mechanisms such as induction of related signaling pathways, effluxion of salt ions, accumulation of these toxic ions in their vacuoles, activation of their detoxification mechanisms and production of osmoprotectans. In this study, identification of salt responsive proteins in moderately halophyte wild type sugar beet Beta vulgaris ssp. maritima was aimed. In order to investigate the protein-based natural stress tolerating mechanisms, plants were exposed to 150 mM NaCl and total proteins were extracted. Differentially expressed proteins were identified by proteomic approaches including MALDI-TOF/TOF mass spectrometry combined two dimensional polyacrylamide gel electrophoresis. The results revealed that enzymatic antioxidants and secondary members of antioxidative pathways are responsive in salt stress. In conclusion, these detected proteins demonstrate that antioxidative system may be the major defense mechanism in halophytic plants.
Subject Term
:
Plant molecular biology.
Plants -- Effect of salt on.
Plant proteomics.
Beets.
Mass spectrometry.
Added Author
:
Karakaya, H. Çağlar Karakaya.
Added Corporate Author
:
İzmir Institute of Technology. Molecular Biology and Genetics.
Added Uniform Title
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics--Thesis (Master).
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Thesis | T001013 | QK728 .C139 2012 | Tez Koleksiyonu |
IYTE Library | Supplementary CD-ROM | ROM1939 | QK728 .C139 2012 EK.1 | Tez Koleksiyonu |