Characterization of outer membrane proteins of salmonella enteritidis in response to phenolic acids stress
by
Özdemir, Özgün Öykü.
Title
:
Characterization of outer membrane proteins of salmonella enteritidis in response to phenolic acids stress
Author
:
Özdemir, Özgün Öykü.
Personal Author
:
Özdemir, Özgün Öykü.
Publication Information
:
[s.l.]: [s.n.], 2012.
Physical Description
:
xii, 44 leaves. : ill. + 1 computer laser optical disc.
Abstract
:
Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) is one of the most reported foodborne pathogen bacterium throughout the world that causes large outbreaks and may result in deaths. Phenolic acids, synthesized in almost all plants, are important for plant metabolism and protection of plants. They can be thought as promising antimicrobial agents against Salmonella Enteritidis. Outer membrane proteins are unique structures to Gram-negative bacteria including Salmonella genus and they are the first targets to environmental changes. This study showed the antimicrobial effect of 3-HPAA, cinnamic acid and o-coumaric acid on Salmonella Enteritidis in vitro. Scanning electron microscopy was used for showing the changes in bacterial shapes. Their effects on the outer membrane protein profile were investigated via proteomic approach. According to results of 96-well microtiter plate assay used for antimicrobial effect determination, among these phenolic acids, 3-HPAA showed 100% growth inhibition and other phenolics retarded the bacterial growth. 30 mM was found as minimum inhibitory concentration (MIC) of 3-HPAA at 24th hour of the experiment. SEM results demonstrated that 3-HPAA causes bacteria to have collapses on cell surface while cinnamic acid and o-coumaric acid cause cells to elongate abnormally. SDS PAGE of the OMPs of bacteria who were exposed to phenolic acids showed changes in amounts of proteins in some protein bands compared to control OMPs. 2-D PAGE results of OMPs of control bacteria via OMPs of 3-HPAA, cinnamic acid and o-coumaric acid treated bacteria, changes in sizes of spots were observed.
Subject Term
:
Salmonella enteritidis.
Phenolic acids.
Membrane proteins.
Added Author
:
Soyer, Ferda.
Added Corporate Author
:
İzmir Institute of Technology. Molecular Biology and Genetics.
Added Uniform Title
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics--Thesis (Master).
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Thesis | T001039 | QR201.S25 O99 2012 | Tez Koleksiyonu |
IYTE Library | Supplementary CD-ROM | ROM1968 | QR201.S25 O99 2012 EK.1 | Tez Koleksiyonu |