Analysis of lysosomal Neu4 sialidase associated proteins by using mass spectrometry (MS/MS)
by
Öztürk, Süleyman Can.
Title
:
Analysis of lysosomal Neu4 sialidase associated proteins by using mass spectrometry (MS/MS)
Author
:
Öztürk, Süleyman Can.
Personal Author
:
Öztürk, Süleyman Can.
Publication Information
:
[s.l.]: [s.n.], 2012.
Physical Description
:
xi, 41leaves. : ill. + 1 computer laser optical disk.
General Note
:
25.01.2014 tarihine kadar tam metin erişimi yazar tarafından kısıtlanmıştır.
Abstract
:
Sialidases are glycohydrolytic enzymes which remove sialic acid residues from glycoproteins, oligosaccharides and glycolipids. There are 4 different sialidases known in mammalians. These are Neu1 (lysosomal), Neu2 (cytoplasmic), Neu3 (cell membrane) and Neu4 (lysosomal/mitochondrial) sialidase. Sialidases are involved in many metabolic and cellular processes interactioning with another proteins or work together in multiprotein complexes. For example, Neu1 is only active with betagalactosidase and cathepsin A enzyme in lysosome. Interactions of sialidases Neu2, Neu3 and Neu4 enzyme with other proteins remain unknown In our study, we aimed to identify proteins which have interaction with sialidase Neu4 as well as Neu1 by using mass spectrometry analysis to find new possible roles of sialidases. Our bait protein's cDNA was tagged with calmodulin binding protein as well as streptavidin binding protein. After transfection and expression of vectors to mammalian cells, proteins were purified using tandem affinity purification (TAP). We identified some associated proteins with sialidase Neu1 and Neu4 by using MS/MS analysis and bioinformatics.
Subject Term
:
Mass spectrometry.
Proteins -- Analysis.
Neuraminidase.
Glycosidases.
Added Author
:
Seyrantepe, Volkan.
Added Corporate Author
:
İzmir Institute of Technology. Molecular Biology and Genetics.
Added Uniform Title
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics.--Thesis (Master).
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Thesis | T001086 | QP551 .O99 2012 | Tez Koleksiyonu |
IYTE Library | Supplementary CD-ROM | ROM2035 | QP551 .O99 2012 EK.1 | Tez Koleksiyonu |