The sustainable packaging solutions require the development of materials based on renewable resources and efficient production methods, which are important nowadays. In this study, a multilayer coated flexible polyethylene terephthalate (PET) sheets were produced by Layer-by-Layer (LbL) assembly using chitosan and pectin bio-based materials incorporated with galls extract (GE) as an active agent. These fabricated films were firstly characterized and results of increasing the depositing of chitosan and pectin from 0 to 60 layers indicated that the deposition of biopolymers successfully improve the performance of flexible films. The optimization of the deposition different number of layer on PET (20 – 40 and 60 layers) was carried out by the study of the surface characterization and the shelf-life tests in order to find out surface modification. The coated LbL films were applied on fresh-cut ‘Abate Fetel’ pears and color, microbial, sensory assays were carried out during 7 days of storage at 4 ºC. At the end of storage, in terms of all tests, the LbL treated samples were presented better preserved compared to the control samples (treated base film). In addition, the release of active agents from multilayer films was evaluated using LbL films in contact with food and gradual reduction was observed. This research demonstrates the feasibility of LbL coating, which acts as a carrier of active substances to enhance the quality of fresh-cut products as an active packaging system.