A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
by
 
Barkley, Courtney

Title
: 
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals

Author
: 
Barkley, Courtney

Personal Author
: 
Barkley, Courtney

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (486 seconds)

Series
: 
Developmental Biology

General Note
: 
Title from resource description page

Abstract
: 
We describe the isolation, dispersion and plating of dental pulp (DP) primary cells with trigeminal (TG) neurons cultured atop overlying transwell filters. Cellular responses of DP cells can be analyzed with immunofluorescence or RNA/protein analysis. Immunofluorescence of neuronal markers with confocal microscopy permits the analysis of neurite outgrowth responses.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Developmental Biology

Electronic Access
: 
https://www.jove.com/t/60809


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2248888-1001XX(2248888.1)JoVe Video Online