HPLC : A Practical Guide.

HPLC A Practical Guide -- Contents -- Chapter 1 Basic Concepts of High-performance Liquid Chromatography -- 1 Physical Parameters for High-speed Separations -- 2 Physical Considerations -- 3 Chemical Influences on the Separation Factor -- 4 Basic Considerations of Liquid Chromatography -- 5 Bibliography -- Chapter 2 Instrumentation -- 1 Chromatographic Systems -- 2 Injectors -- 3 Eluent Delivery Systems -- Composition Gradient Delivery Systems -- Pressure Drop Changes due to Changes in the Eluent Composition -- Flow Rate Gradients -- Step-wise Elution -- Recycle Elution -- Column Switching Separation -- 4 Degassing Methods -- 5 Column Temperature Control -- 6 Detectors -- Spectroscopic Detectors -- Electrochemical Detectors -- Mass Spectrometer -- Other Detectors -- Measurement of Detector Sensitivity -- 7 Recording Systems -- Selection of Detector Time Constant -- 8 Columns and Connectors -- 9 Flow Cell Volume and Connecting Tube Dimensions for High Efficiency Operation -- 10 Other Components in a Liquid Chromatograph -- 11 Trouble-shooting -- 12 References -- Reference Books -- Chapter 3 Preparation, Testing, and Selectivity of Stationary Phase Materials -- 1 Synthesis of Stationary Phase Materials -- Silica Gel -- Surface Modification of Silica Gel -- Modification Using a Halogenated Silica Surface -- Modification Using a Chlorosilane -- Synthesis of Ion-exchanger Stationary Phases -- Cation-exchanger -- Anion-exchanger -- Organic Stationary Phase Materials -- 2 Sieving of Stationary Phase Materials -- 3 Column Packing Methods -- 4 Column Evaluation -- Column Efficiency and Asymmetry -- Column Test for Reversed-phase Liquid Chromatography -- Inertness Test for Basic Compounds -- Inertness Test for Acidic Compounds -- Inertness Test for Heavy Metals -- Physicochemical Tests of the Stationary Phase -- 5 Measurement of Void Volume.

Elution Volume of Proposed Void Volume Markers -- 6 Selectivity of Stationary Phase Materials -- Silica Gel-based Stationary Phase Materials -- Organic Polymer-based Stationary Phase Materials -- Other Stationary Phase Materials -- 7 References -- Chapter 4 Selection of the Eluent -- 1 Reversed-phase Liquid Chromatography -- Selectivity of Organic Modifiers in the Eluent -- Comparison of the Effect of Organic Modifiers on Analytes with log P = 3 -- Chromatographic Behaviour of log P = 3 Compounds in Aqueous Tetrahydrofuran -- Chromatographic Behaviour of log P = 3 Compounds in Aqueous Methanol -- Chromatographic Behaviour of log P = 3 Compounds in Aqueous Acetone -- Chromatographic Behaviour of log P = 3 Compounds in Aqueous Dimethylformamide (DMF) -- Comparison of Effects of Modifiers on a Mixture of Analytes -- 2 Chromatography of Ionic Compounds -- pH Effects -- Ion-pair Separations -- Theory of Ion-pair Liquid Chromatography -- Normal Phase Ion-pair Partition Liquid Chromatography -- Reversed-phase Ion-pair Liquid Chromatography -- pH Effects -- Effect of the Ionic Strength of the Buffer Solution -- Selectivity of Organic Modifier and the Concentration Effect -- Column Temperature Effects -- Detection -- Trouble-shooting in Ion-pair Liquid Chromatography -- 3 Normal-phase Liquid Chromatography -- Classification of Solvents -- Preparation of the Eluent -- 4 Size-exclusion Liquid Chromatography (SEC) -- Aqueous Phase Size-exclusion -- Organic Phase Size-exclusion -- 5 References -- Bibliography of additional references on theoretical approaches in ion-pair liquid chromatography -- Chapter 5 Separation Based on an Improved Column Efficiency -- 1 Improving Separation by Changing the Selectivity -- 2 Improving Separation by Increasing the Column Efficiency -- Reducing the Eddy Diffusion Term -- Reducing the Effect of Longitudinal Diffusion.

Reducing the Effect of Resistance to Mass Transport in the Stationary and Mobile Phases -- 3 Bibliography -- Chapter 6 Influence of Physical Chemistry on Separations in Liquid Chromatography -- 1 Quantitative Structure-Retention Relationships in Reversed-phase Liquid Chromatography -- Prediction of Retention Times from log P in Reversed-phase Liquid Chromatography -- Prediction of Partition Coefficient (log P) -- Correlation between log P and log k Values -- Prediction of Retention Times from log P -- Prediction of Retention Time of Ionic Compounds from log P and pKa -- Calculation of Dissociation Constant pKa from Hammet's Equation -- 2 Van der Waals Volume as the Basic Property -- Calculation of van der Waals Volume -- Prediction of Retention Times Based on van der Waals Volumes -- 3 Enthalpy as a Thermodynamic Property in Retention Studies -- Measurement of Enthalpy by Liquid Chromatography -- Enthalpy Related to Retention -- 4 Conclusion -- 5 References -- Subject Index.