Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
Title:
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
Author:
Shaikh Qureshi, Wasay M.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (450 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54303