Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
Title:
Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
Author:
Li, Hailong
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (613 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53697