Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
Title:
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
Author:
Lombardo, Verónica A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (600 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52087