Cover -- Contents of Volume 1 -- Contents of other Volumes -- Contributors -- Preface -- PART A: CELL AND TISSUE CULTURE: ASSORTED TECHNIQUES -- SECTION 1: General Techniques -- CHAPTER 1: Setting up a Cell Culture Laboratory -- CHAPTER 2: General Procedures for Cell Culture -- CHAPTER 3: Counting Cells -- CHAPTER 4: Cell Proliferation Assays: Improved Homogeneous Methods Used to Measure the Number of Cells in Culture -- CHAPTER 5: Development of Serum-Free Media: Optimization of Nutrient Composition and Delivery Format -- CHAPTER 6: Cell Line Authentication -- CHAPTER 7: Detection of Microbial and Viral Contaminants in Cell Lines -- SECTION 2: Culture of Specific Cell Types: Stem Cells -- CHAPTER 8: Neural Crest Stem Cells -- CHAPTER 9: Postnatal Skeletal Stem Cells: Methods for Isolation and Analysis of Bone Marrow Stromal Cells from Postnatal Murine -- CHAPTER 10: Establishment of Embryonic Stem Cell Lines from Adult Somatic Cells by Nuclear Transfer -- CHAPTER 11: T-Cell Isolation and Propagation in vitro -- CHAPTER 12: Generation of Human and Murine Dendritic Cells -- SECTION 3: Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial -- CHAPTER 13: Clonal Cultures in vitro for Haemopoietic Cells Using Semisolid Agar Medium -- CHAPTER 14: Human Skeletal Myocytes -- CHAPTER 15: Growing Madin-Darby Canine Kidney Cells for Studying Epithelial Cell Biology -- CHAPTER 16: Cultivation and Retroviral Infection of Human Epidermal Keratinocytes -- CHAPTER 17: Three-Dimensional Cultures of Normal and Malignant Human Breast Epithelial Cells to Achieve in vivo-like Architecture and Function -- CHAPTER 18: Primary Culture of Drosophila Embryo Cells -- CHAPTER 19: Laboratory Cultivation of Caenorhabditis elegans and Other Free-Living Nematodes -- SECTION 4: Differentiation and Reprogramming of Somatic Cells.

CHAPTER 20: Induction of Differentiation and Cellular Manipulation of Human Myeloid HL-60 Leukemia Cells -- CHAPTER 21: Cultured PC12 Cells: A Model for Neuronal Function, Differentiation, and Survival -- CHAPTER 22: Differentiation of Pancreatic Cells into Hepatocytes -- CHAPTER 23: TERA2 and Its NTERA2 Subline: Pluripotent Human Embryonal Carcinoma Cells -- CHAPTER 24: Embryonic Explants from Xenopus laevis as an Assay System to Study Differentiation of Multipotent Precursor Cells -- CHAPTER 25: Electrofusion: Nuclear Reprogramming of Somatic Cells by Cell Hybridization with Pluripotential Stem Cells -- CHAPTER 26: Reprogramming Somatic Nuclei and Cells with Cell Extracts -- SECTION 5: Immortalization -- CHAPTER 27: Immortalization of Primary Human Cells with Telomerase -- CHAPTER 28: Preparation and Immortalization of Primary Murine Cells -- SECTION 6: Somatic Cell Hybrids -- CHAPTER 29: Viable Hybrids between Adherent Cells: Generation, Yield Improvement, and Analysis -- SECTION 7: Cell Separation Techniques -- CHAPTER 30: Separation and Expansion of Human T Cells -- CHAPTER 31: Separation of Cell Populations Synchronized in Cell Cycle Phase by Centrifugal Elutriation -- CHAPTER 32: Polychromatic Flow Cytometry -- CHAPTER 33: High-Speed Cell Sorting -- SECTION 8: Cell Cycle Analysis -- CHAPTER 34: Cell Cycle Analysis by Flow and Laser-Scanning Cytometry -- CHAPTER 35: Detection of Cell Cycle Stages in situ in Growing Cell Populations -- CHAPTER 36: In vivo DNA Replication Labeling -- CHAPTER 37: Live Cell DNA Labeling and Multiphoton/Confocal Microscopy -- SECTION 9: Cytotoxic and Cell Growth Assays -- CHAPTER 38: Cytotoxicity and Cell Growth Assays -- CHAPTER 39: Micronuclei and Comet Assay -- SECTION 10: Apoptosis -- CHAPTER 40: Methods in Apoptosis -- SECTION 11: Assays of Cell Transformation, Tumorigenesis, Invasion, and Wound Healing.

CHAPTER 41: Cellular Assays of Oncogene Transformation -- CHAPTER 42: Assay of Tumorigenicity in Nude Mice -- CHAPTER 43: Transfilter Cell Invasion Assays -- CHAPTER 44: Endothelial Cell Invasion Assay -- CHAPTER 45: Analysis of Tumor Cell Invasion in Organotypic Brain Slices Using Confocal Laser-Scanning Microscopy -- CHAPTER 46: Angiogenesis Assays -- CHAPTER 47: Three-Dimensional, Quantitative in vitro Assays of Wound Healing Behavior -- SECTION 12: Electrophysiological Methods -- CHAPTER 48: Patch Clamping -- SECTION 13: Organ Cultures -- CHAPTER 49: Preparation of Organotypic Hippocampal Slice Cultures -- CHAPTER 50: Thyroid Tissue-Organotypic Culture Using a New Approach for Overcoming the Disadvantages of Conventional Organ Culture -- PART B: VIRUSES -- SECTION 14: Growth and Purification of Viruses -- CHAPTER 51: Growth of Semliki Forest Virus -- CHAPTER 52: Design and Production of Human Immunodeficiency Virus-Derived Vectors -- CHAPTER 53: Construction and Propagation of Human Adenovirus Vectors -- CHAPTER 54: Production and Quality Control of High-Capacity Adenoviral Vectors -- CHAPTER 55: Novel Approaches for Production of Recombinant Adeno-Associated Virus -- PART C: ANTIBODIES -- SECTION 15: Production and Purification of Antibodies -- CHAPTER 56: Production of Peptide Antibodies in Rabbits and Purification of Immunoglobulin -- CHAPTER 57: Preparation of Monoclonal Antibodies -- CHAPTER 58: Rapid Development of Monoclonal Antibodies Using Repetitive Immunizations, Multiple Sites -- CHAPTER 59: Phage-Displayed Antibody Libraries -- CHAPTER 60: Ribosome Display: In Vitro Selection of Protein-Protein Interactions -- CHAPTER 61: Epitope Mapping by Mass Spectrometry -- CHAPTER 62: Mapping and Characterization of Protein Epitopes Using the SPOT Method -- CHAPTER 63: Determination of Antibody Specificity by Western Blotting.

CHAPTER 64: Enzyme-Linked Immunosorbent Assay -- CHAPTER 65: Radioiodination of Antibodies -- PART D: IMMUNOCYTOCHEMISTRY -- SECTION 16: Immunofluorescence -- CHAPTER 66: Immunofluorescence Microscopy of Cultured Cells -- CHAPTER 67: Immunofluorescence Microscopy of the Cytoskeleton: Combination with Green Fluorescent Protein Tags -- CHAPTER 68: Immunocytochemistry of Frozen and of Paraffin Tissue Sections -- PART E: Appendix -- CHAPTER 69: Representative Cultured Cell Lines and Their Characteristics.