A Low-Cost Approach to PCR : Appropriate Transfer of Biomolecular Techniques.
Title:
A Low-Cost Approach to PCR : Appropriate Transfer of Biomolecular Techniques.
Author:
Harris, Eva.
ISBN:
9780198028017
Personal Author:
Physical Description:
1 online resource (327 pages)
Contents:
Contents -- Foreword -- I. PCR AND TECHNOLOGY TRANSFER -- 1 Introduction -- 1.1. Molecular Biology and the Polymerase Chain Reaction -- 1.2. Infectious Diseases and International Health -- 1.3. Appropriate Application of Technology -- 1.4 General Principles -- 1.5. Book Structure -- 2 PCR Technology -- 2.1. Description of the Technique -- 2.2. Technical Details -- 2.3. Frequently Used PCR-Based Techniques -- 2.4. Applications -- 3 Principles of Sustainable Technology Transfer -- 3.1. A Low-Cost Methodology -- 3.2. Knowledge-Based Participatory Transfer Process -- 3.3. Appropriate Application -- 3.4. Intraregional Cooperation -- 4 Case Study: The AMB/ATT Program -- 4.1. Introduction -- 4.2. Program Description -- 4.3. Program Structure -- 4.4. Program Development -- 4.5. Examples of Projects -- 4.6. International Courses -- II. SELECTED PROTOCOLS -- 5 Infectious Disease Protocols -- 5.1. PCR Protocols -- 5.2. Nonradioactive DNA Probes: V. cholerae Colony Blot -- 6 Rapid Cloning of PCR Products -- 6.1. Primer Design -- 6.2. Preparation of PCR Products for Cloning -- 6.3. Ligation -- 6.4. Preparation of Competent Cells and Transformation -- 6.5. Checking Clones by PCR -- 6.6. Plasmid Purification -- 6.7. Analysis of Clones by Restriction Enzyme Digestion -- 6.8. Agarose Gel Electrophoresis -- Appendix A: Construction of Laboratory Equipment -- Appendix B: In-House Preparation of Reagents -- B.1. Useful Formulas -- B.2. Solutions -- B.3. Preparation of Selected Reagents -- B.4. DNA Size Markers -- Appendix C: Inventory for a PCR Laboratory -- Appendix D: Good Laboratory Practice -- D.1. General Tips -- D.2. Calibration of Adjustable Pipettors -- Appendix E: Prevention of Cross-Contamination -- Appendix F: PCR Troubleshooting Guide and Flowchart -- Appendix G: Workshop Organization and Teaching Tips -- Appendix H: Sample Charts and Worksheets.
Appendix I: Useful World Wide Web Sites -- Glossary -- A -- B -- C -- D -- E -- F -- G -- H -- I -- K -- L -- M -- N -- O -- P -- R -- S -- T -- U -- V -- Afterword -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- K -- L -- M -- N -- P -- R -- S -- T -- V -- W -- U -- Y -- Z.
Abstract:
The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.
Local Note:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
Genre:
Electronic Access:
Click to View