Contents -- Preface -- Acknowledgements -- PART I -- 1 Introduction -- 1.1. Seed-transmitted Viruses and Viroids -- 1.2. The Development of the Science -- 1.3. Economic Importance of Seed-transmitted Viruses -- 1.4. Testing of Seeds for Viruses: Why? -- References -- 2 Seed Transmission of Viruses -- 2.1. Location of Inoculum in the Seed -- 2.2. Plant-to-seed and Seed-to-plant Transmission -- References -- 3 Ecology, Epidemiology and Control -- 3.1. Ecology and Epidemiology -- 3.2. Control Strategies -- References -- PART II -- 4 Biological Assays -- 4.1. Symptomatology -- 4.2. Facilities and Equipment for Biological Assays -- 4.3. Growing-on Tests -- 4.4. Infectivity Assays -- 4.5. Maintenance of Isolates -- References -- 5 Serological Testing Methods -- 5.1. Antigens and Antibodies -- 5.2. Enzyme-linked Immunosorbent Assay (ELISA) -- 5.3. Dot Immunobinding Assay (DIBA) -- 5.4. Tissue Blotting Immunoassay (TBIA) -- 5.5. Other Serological Test Methods -- References -- 6 Nucleic Acid-based Testing Methods -- 6.1. Nucleic Acid Hybridization -- 6.2. Enzymatic Nucleic Acid Amplification -- References -- 7 Epilogue -- 7.1. Other Detection Techniques -- 7.2. Organization and Interpretation of Seed-health Assays -- 7.3. Tolerance Levels of Infection, and Pathogens in Germplasm -- 7.4. Standardization and Cost of Tests -- References -- Appendix 1. List of Seed-transmitted Viruses and Viroids -- Appendix 2. Reagents, Solutions and Buffers -- Appendix 3. Suppliers of Laboratory Equipment and Materials -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- Y -- Z.