Front Cover -- Insect Molecular Genetics -- Copyright Page -- CONTENTS -- Preface to the Second Edition -- Preface to the First Edition -- Part I: Genes and Gemome Organization in Eukaryotes -- Chapter 1. DNA, Gene Structure, and DNA Replication -- 1.1. Overview -- 1.2. Introduction to the Central Dogma -- 1.3. The "RNA World" Came First? -- 1.4. The Molecular Structure of DNA -- 1.5. The Molecular Structure of RNA -- 1.6. The Double Helix -- 1.7. Complementary Base Pairing Is Fundamental -- 1.8. DNA Exists in Several Forms -- 1.9. Genes -- 1.10. The Genetic Code Is a Triplet and Is Degenerate -- 1.11. Gene Organization -- 1.12. Efficient DNA Replication Is Essential -- 1.13. DNA Replication Is Semiconservative -- 1.14. Replication Begins at Replication Origins -- 1.15. Replication Occurs Only in the 5' to 3' Direction -- 1.16. Replication of DNA Requires an RNA Primer -- 1.17. Ligation of Replicated DNA Fragments -- 1.18. DNA Replication in Eukaryotes -- 1.19. Telomeres at the End: A Solution to the Loss of DNA during Replication -- 1.20. DNA Replication Fidelity and DNA Repair -- 1.21. Mutations in the Genome -- 1.22. Common Conventions in Genetic Terminology -- 1.23. Independent Assortment and Recombination During Sexual Reproduction -- General References -- References Cited -- Chapter 2. Transcription, Translation, and Regulation of Eukaryotic DNA -- 2.1. Overview -- 2.2. Introduction -- 2.3. RNA Synthesis Is Gene Transcription -- 2.4. Transcription Involves Binding, Initiation, Elongation, and Termination -- 2.5. RNA Transcripts Are Longer Than the Protein-Coding Gene -- 2.6. RNA of Protein-Coding Genes Must Be Modified and Processed in Eukaryotes -- 2.7. Splicing Out the Introns -- 2.8. Translation Involves Protein Synthesis -- 2.9. mRNA Surveillance: Damage Control -- 2.10. Import and Export from the Nucleus.

2.11. Transport of Proteins within the Cytoplasm -- 2.12. mRNA Stability -- 2.13. Chaperones and the Proteosome -- 2.14. RNA Silencing or Interference -- 2.15. Gene Regulation in Eukaryotes -- 2.16. Insulators and Boundaries -- 2.17. Chromosome or Gene Imprinting by Methylation in Insects? -- 2.18. Eukaryotic Genomes and Evolution -- General References -- References Cited -- Chapter 3. Nuclear and Extranuclear DNA in Insects -- 3.1. Overview -- 3.2. Introduction -- 3.3. C Value Paradox -- 3.4. Repetitive DNA Is Common in Insects -- 3.5. Composition of Insect DNA -- 3.6. Chromosomes Are DNA plus Proteins -- 3.7. Packaging Long, Thin DNA Molecules into Tiny Spaces -- 3.8. Structure of the Nucleus -- 3.9. Euchromatin and Heterochromatin -- 3.10. Centromeres -- 3.11. Telomeres -- 3.12. Chromosomes during Mitosis and Meiosis -- 3.13. Chromosome Damage -- 3.14. Polyteny -- 3.15. Chromosomal Puffing -- 3.16. B Chromosomes -- 3.17. Sex Chromosomes -- 3.18. Extranuclear Inheritance in Mitochondrial Genes -- 3.19. Transposable Elements Are Ubiquitous Agents That Alter Genomes -- References Cited -- Chapter 4. Genetic Systems, Genome Evolution, and Genetic Control of Embryonic Development in Insects -- 4.1. Overview -- 4.2. Introduction -- 4.3. Genetic Systems in Insects -- 4.4. Endopolyploidy Is Common in Somatic Tissues of Arthropods -- 4.5. Genetics of Insects Other Than D. melanogaster -- 4.6. Dynamic Insect Genomes -- 4.7. B Chromosomes -- 4.8. Unique-Sequence DNA in the Nucleus -- 4.9. Middle-Repetitive DNA in the Nucleus -- 4.10. Highly Repetitive DNA -- 4.11. Producing Large Amounts of Protein in a Short Time: Gene Amplification and Gene Duplication -- 4.12. Multiple Genomes in Insects: What Is the "Biological Individual?" -- 4.13. Insect Development -- 4.14. Dissecting Development with D. melanogaster Mutants -- 4.15. Interactions during Development.

4.16. Similarities and Differences in Development in Other Insects -- 4.17. Evo-Devo and the Revolution in Developmental Studies -- References Cited -- Some Relevant Web Sites -- Part II: Molecular Genetic Techniques -- Chapter 5. Some Basic Tools: How to Cut, Paste, Copy, Measure, and Visualize DNA -- 5.1. Overview -- 5.2. Introduction to a Simple Experiment -- 5.3. Extracting DNA -- 5.4. Precipitating Nucleic Acids -- 5.5. Shearing DNA -- 5.6. Cutting DNA with Restriction Endonucleases -- 5.7. Joining DNA Molecules -- 5.8. Growth, Maintenance, and Storage of E. coli -- 5.9. Plasmids for Cloning in E. coli -- 5.10. Transforming E. coli with Plasmids -- 5.11. Purifying Plasmid DNA from E. coli -- 5.12. Electrophoresis in Agarose and Acrylamide Gels -- 5.13. Detecting, Viewing, and Photographing Nucleic Acids in Gels -- 5.14. Identifying DNA by Southern Blot Analysis -- 5.15. Labeling DNA or RNA Probes -- 5.16. Removing DNA from Agarose Gels after Electrophoresis -- 5.17. Restriction Site Mapping -- General References -- References Cited -- Some Relevant Web Sites -- Chapter 6. Cloning and Expression Vectors, Libraries, and Their Screening -- 6.1. Overview -- 6.2. Introduction -- 6.3. The Perfect Genomic Library -- 6.4. cDNA Cloning -- 6.5. Enzymes Used in Cloning -- 6.6. Isolating a Specific Gene from a Library -- 6.7. Labeling Probes by a Variety of Methods -- 6.8. Baculovirus Vectors for Expressing Foreign Polypeptides in Insect Cells -- General References -- Some Relevant Web Sites -- Chapter 7. DNA Sequencing and the Evolution of the "-Omics" -- 7.1. Overview -- 7.2. Introduction -- 7.3. The Dideoxy or Chain-Terminating Method -- 7.4. Variations on Dideoxy Sequencing Methods -- 7.5. DNA Sequences Can Be Analyzed on Polyacrylamide Gels -- 7.6. Sequencing Reactions Require a Primer -- 7.7. The Maxam and Gilbert Sequencing Method.

7.8. Shotgun Strategies for Genomes -- 7.9. Sequencing DNA by the Polymerase Chain Reaction (PCR) -- 7.10. Automated DNA Sequencers -- 7.11. Analyzing DNA Sequence Data -- 7.12. DNA Sequence Data Banks -- 7.13. A Brief History of the Drosophila Genome Project -- 7.14. Bioinformatics -- 7.15. Genome Analyses of Other Arthropods -- 7.16. TEs as Agents of Genome Evolution -- 7.17. DNA Microarrays, Gene Chips, and a Laboratory-on-a-Chip -- 7.18. Proteomics: Another "-Omic" -- 7.19. Functional Genomics -- 7.20. Structural Genomics: Another New Horizon? -- 7.21. Comparative Genomics -- 7.22. The Post-Genomic Era: Reductionism Gives Way to Emergent Properties? -- Reference Cited -- Some Relevant Web Sites -- Chapter 8. DNA Amplification by the Polymerase Chain Reaction: Molecular Biology Made Accessible -- 8.1. Overview -- 8.2. Introduction -- 8.3. The Basic Polymerase Chain Reaction (PCR) -- 8.4. Some Modifications of the PCR -- 8.5. Some Research Applications -- 8.6. Concluding Remarks -- References Cited -- Chapter 9. Transposable-Element Vectors to Transform Drosophila and Other Insects -- 9.1. Overview -- 9.2. Introduction -- 9.3. P Elements and Hybrid Dysgenesis -- 9.4. P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7. P Vectors and Germ-Line Transformation -- 9.8. Using P-Element Vectors -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects Other Than Drosophila -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity.

9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- General References -- References Cited -- Part III: Applications in Elntomology -- Chapter 10. Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.6. Are Sex Determination Mechanisms Diverse? -- 10.7. A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.12. A Mite Consisting Only of Haploid Females -- 10.13. Paternal Sex Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.14. Male Killing in Coccinellidae -- 10.15. Sex and the Sorted Insects -- References Cited -- Some Relevant Web Sites -- Chapter 11. Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.5. Molecular Genetic Analyses of Insect Behavior -- 11.6. Human Neurodegenerative Diseases and Addictions in Drosophila -- References Cited -- Chapter 12. Molecular Systematics and Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.5. Targets of DNA Analysis -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.7. The Universal Tree of Life -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.10. Molecular Evolution and Speciation -- Some Relevant Journals -- Reference Cited -- Some Relevant Web Sites -- Chapter 13. Insect Population Ecology and Molecular Genetics.

13.1. Overview.