CONTENTS -- Preface -- PART 1. BIOLUMINESCENCE -- Bioluminescence and mating behaviour in pony fish, Leiognathus nuchalis Azuma N, Furubayashi C, Shichiri T, Wada M, Mizuno N and Suzuki Y -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS -- DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Studies on the bioluminescent mechanism of symplectin photoprotein Isobe M, Matsuda T, Kuse M, Mori H, Fujii T, Kondo N and Kageyama Y -- INTRODUCTION -- RESULTS AND DISCUSSION -- Symplectin analysis -- Proof of chromophoric structure -- Fluorinated dehydrocoelenterazine -- Photoaffinity Labeling -- ACKNOWLEDGEMENTS -- REFERENCES -- Chemical studies on bioluminescence of Acorn worm, Ptychodera flava: isolation and characterization of luminous substances Kanukubo A, Koga K and Isobe M -- INTRODUCTION -- MATERIALS AND METHODS -- Instrumentation -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- pH regulation of luciferase activity in dinoflagellates involves a novel enzymatic mechanism Liu L, Schultz W and Hastings JW -- INTRODUCTION -- THREE ACTIVE SITES IN A SINGLE PROTEIN -- CONSERVED HISTIDINES -- The four conserved histidines are found in all seven species examined -- Structure of domain 3 of L. polyedrum giving the location of histidines -- MOLECULAR MECHANISM OF pH REGULATION -- REFERENCES -- Bioluminescence in Ophiuroids (Echinodermata): a minreview Mallefet J -- LUMINESCENCE IN ECHINODERMS -- LUMINESCENCE IN OPHIUROIDS -- ACKNOWLEDGMENTS -- REFERENCES -- Luminescent behaviour in the New Zealand Glowworm, Arachnocarnpa luminosa (Insecta -- Diptera -- Mycetophilidae) Ohba Nand Meyer-Rochow VB -- INTRODUCTION -- MATERIAL AND METHODS -- Recordings -- Habitat -- RESULTS -- Population density, growth stages, and glowworm nests -- Larval movements in the nest -- Luminescence activity -- Cessation of luminescence -- DISCUSSION.

Prey items in the native habitat -- Larval population densities and growth stages -- Larval nest and fishing lines -- Luminescence activity -- Luminescence stoppages -- ACKNOWLEDGEMENTS -- REFERENCES -- Aequorin and GFP: an historical account Shimomura O -- REFERENCES -- Bioluminescence reaction in the firefly squid Watasenia scintillans Tsuji FI -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGMENTS -- REFERENCES -- Pharmacological and electrophysiological studies of light emission in 3 ophiuroid species: preliminary results Vanderlinden C, Vanhemelen M, Nilius B, Gailly P and Mallefet J -- INTRODUCTION -- METHODS -- RESULTS -- Pharmacology -- Electrophysiology -- DISCUSSION -- ACKNOWLEDGMENTS -- REFERENCES -- PART 2. BEETLE BIOLUMINESCENCE -- Importance of firefly luciferase C-terminal domain in binding of luciferyl-adeny late Ayabe K, Zuko T and Ueda H -- INTRODUCTION -- METHODS -- General methods -- Detection of LH2-AMP -- RESULTS -- Concentration dependency of the N-domain activity -- Sensitive detection of LHz-AMP using the N-domain -- DISCUSSION -- REFFERENCES -- Comparison of kinetic properties of firefly luciferase from Photinus pyralis and Luciola mingrelica Brovko LY, Gandelman OA, Kershengolz IB and Ugarova NN -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS -- Dependence of product yield on enzyme and substrate concentration -- REFERENCES -- Firefly luciferase and Drosophila CG6178 gene product are fatty acyl-CoA synthetases Oba Y, Ojika M and Inouye S -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Solvent effect on the NMR and absorption spectra of firefly luciferin in tetrahydrofuran Odai K, Nishiyama S, Shibata R, Yoshida Y and Wada N -- INTRODUCTION -- EXPERIMENTAL METHODS -- COMPUTATIONAL METHODS -- RESULTS AND DISCUSSION -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES.

An evolutionary history of the Japanese aquatic fireflies inferred from mitochondrial DNA sequences Suzuki H, Sato Y and Ohba N -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Bioluminescence spectra of native and mutant firefly luciferases as a function of pH Ugarova NN, Maloshenok LG and Uporov IV -- INTRODUCTION -- METHODS -- RESULTS -- ACKNOWLEDGMENTS -- REFERENCES -- Interaction of oxyluciferin analogs, dimethyl oxyluciferin and monomethyl oxyluciferin, with firefly luciferase Vlasova TN, Leontieva OV and Ugarova NN -- INTRODUCTION -- METHODS -- RESULTS -- Absorption and fluorescence spectra of MMOL -- Stability of DMOL and MMOL in buffer solutions -- Interaction of MMOL and DMOL with the wild-type and mutant luciferases -- ACKNOWLEDGMENTS -- REFERENCES -- PART 3. MARINE BACTERIA BIOLUMINESCENCE -- Effect of oxygen and hydrogen ion on the modulation of the bioluminescence from luminous bacteria Karatani H, Yoshizawa S and Hirayama S -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Kinetic investigation of bacterial luciferase Mezhevikin W, Sukovataya IE and Tyulkova NA -- INTRODUCTION -- THEORY -- ACKNOWLEDGEMENTS -- REFERENCES -- A MNDO-PM5 study of the enzyme-free NADH generation from NAD' in the presence of electron-transfer mediator and an evaluation of the process based on bacterial bioluminescence Sugimoto T, Wada Nand Karatani H -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Effects of organic solvents on bioluminescence emission spectra of bacterial luciferase from Photobacterium leiognathi Sukovataya IE and Tyulkova NA -- INTRODUCTION -- METHODS -- RESULTS -- ACKNOWLEDGEMENTS -- REFERENCES.

Formation of Hz02 in bacterial bioluminescence reaction with flavinmononucleotide activated with N-methylimidazole on the phosphate group without addition of the exogenous aldehyde Tyulkova NA and Krasnova OI -- INTRODUCTION -- METHODS -- RESULTS -- REFERENCES -- Luminous bacteria: biotechnological aspects Vydryakova GA, Chugaeva YuV, Tyulkova NA, Medvedeva SE, Kuznetsov AM and Rodicheva EK -- INTRODUCTION -- LUMINOUS BACTERIA AS PRODUCERS OF ENZYMES -- DATABASE OF CULTURE COLLECTION IBSO -- REFERENCES -- Expulsion of symbiotic luminous bacteria from pony fish, Leiognathus nuchalis Wada M, Barbara G, Mizuno N, Azuma N, Kogure K and Suzuki Y -- INTRODUCTION -- MATERIALS AND METHODS -- Collection and maintenance of Leiognathus nuchalis -- Experiments with L nuchalis infected by symbiotic luminous P. leiognathi -- Bacterial numbers from SW, fecal matter and juvenile fish LO -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- RFLP analysis of the Lux A genes of Photobacterium leiognathi isolates derived from the symbiotic light organ of Leiognathid fish, Leiognathus rivulatus Wada M, Kamiya A, Kita-Tsukamoto K, Ikejima K, Nishida M and Kogure K -- INTRODUCTION -- METHODS -- Isolation of P. leiognathi from the light organ of L. rivulatus -- DNA extraction and PCR amplification of the luxA gene of P. leiognathi -- Sequencing and RFLP analysis of the luxA genes -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Supplements for Photobacterium phosphoreum RL- 1 culture medium to enhance the luminescence activity Yu R, Imada C, Wada M, Kobayashi T, Hamaah-Sat0 Nand Watanabe E -- INTRODUCTION -- METHODS -- Strains and culture conditions -- Preparation of supplements -- Culture and measurement of luminescence activity -- RESULTS -- Effect of marine food extracts on RLU of RL-1 -- Effect of amino acids and chitosan on RLU of RL-1.

Effect of amino acids and chitosan on RLU of other strains -- ACKNOWLEDGEMENT -- REFERENCES -- PART 4. CYPRIDINA (VARGULA) BIOLUMINESCENCE -- Superoxide or singlet oxygen: the chemiluminescence of Cypridina luciferin analogues in photodynamic solutions Bancirova M and Snyrychova I -- INTRODUCTION -- MATERIAL AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- ACKNOWLEDGMENTS -- REFERENCES -- Development of the chemistry of the imidazopyrazionone-bioluminescence system: From the bio- and chemiluminescence mechanism to a design of sensor molecules Hirano T, Nakai S, Sekiguchi T, Fuji0 S, Maki S and Niwa H -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Biosynthesis of Cypridina luciferin from free amino acids in Cypridina (Vargula) hilgendotjii Kato S, Oba Y, Ojika M and Inouye S -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Studies on the chemiluminescence mechanism of Cypridina luciferin analogues: dissociation constants of the singlet-excited Cypridina oxyluciferin analogues Saito R, Iwasa E and Katoh A -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSION -- REFERENCES -- Biosynthesis of Vargula hilgendofii luciferin, arisen from L-arginine, L-tryptophan, and L-isoleucine Toya Y -- INTRODUCTION -- METHODS -- Animals -- Materials -- Complete release of luciferin (1) from Vargula by electric stimulation -- Preparation of the formula bait -- Feeding experiment -- Extraction and purification of etioluciferin (3) from the spent seawater -- Instrumentation -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- PART 5. CHEMILUMINESCENCE -- On the role of the singlet-oxygen dimol chemiluminescence in dioxirane reactions Adam W, Kazakov VP, Kazakov DV, Lutypova RR, Maistrenko GY, Mal'zev DV and Safarov FE -- INTRODUCTION.

EXPERIMENTAL SECTION.