Contents -- Preface -- List of Figures -- List of Contributors -- A Protein Purification -- Summary -- A.1 Protein Precipitation -- A.2 Column Chromatography -- A.2.1 Ionic Exchange Chromatography -- A.2.2 Size-Exclusion Chromatography -- A.2.3 Affinity Chromatography -- A.2.4 Purification of Recombinant Proteins -- A.2.5 Commercially Prepacked Column Kits -- Notes -- B Protein Analysis -- B.1 Protein Estimation -- B.1.1 Absorbance Assays -- B.1.2 Colourimetric Assays -- B.1.3 Commercial Protein Estimation Kits -- B.2 Spectrometric Analysis -- B.3 SDS-PAGE -- B.4 Gel Staining -- B.4.1 Coomassie Blue Staining -- B.4.2 Silver Staining -- B.5 Western Blotting -- B.6 Immunoprecipitations -- Notes -- C Lipid Analysis -- C.1 Lipid Extraction -- C.1.1 Modified Bligh and Dyers Method for Phospholipid Extraction -- C.1.2 Folch Extraction -- C.1.3 Grey Method for Phosphatidylinositol Phosphate Extraction -- C.1.4 Modified Alex Brown Method for Phosphatidylinositol Phosphate Extraction -- C.1.5 Hexane Extraction for Neutral Lipids -- C.1.6 Glycolipid Extraction -- C.2 Thin Layer Chromatography -- Notes -- D Mammalian Cell Culture -- D.1 Primary Culture -- D.1.1 Primary Cell Isolation and Culturing -- D.1.2 Splenocyte Isolation -- D.1.3 Isolation of Peripheral Blood Lymphocytes -- D.2 Tissue Culture -- D.2.1 Adherent Cells -- D.2.2 Suspension Cells -- D.2.3 Cell Maintenance -- D.3 Cell Count -- D.4 Mammalian Transfection -- D.4.1 Calcium Phosphate Coprecipitation -- D.4.2 Electroporation Protocol -- D.4.3 Lipofectamine Transfection Protocol -- Notes -- E Microscopy -- E.1 Poly-L-Lysine Immobilisation -- E.2 Immunofluorescence and Confocal Microscopy -- E.3 Immunohistochemistry -- Notes -- F Assays -- F.1 Enzyme Assays -- F.1.1 Enzyme Kinetics: Basics -- F.1.2 Lipid Kinase Assay -- F.1.3 Protein Kinase Assay -- F.1.4 Protein Tyrosine Phosphatase Assay.

F.1.5 Alkaline Phosphatase Assay -- F.1.6 Caspase Assay -- F.2 Functional Assays -- F.2.1 Apoptosis Assay -- F.2.2 XTT Cell Proliferation Assay [(2,3-Bis (2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide)] -- F.2.3 Chemotaxis Assay -- F.2.4 Matrigel Invasion or Chemoinvasion Assay -- Notes -- G Autoradiography -- Summary -- Notes -- Suggested Readings -- Abbreviations -- Appendices -- Appendix 1 Safety Issues -- Appendix 2 Buffer Chart -- Appendix 3 Composition of Regular Buffers or Stock Solutions -- Index.