Front Cover -- Principles of Regenerative Medicine -- Copyright Page -- Contents -- Preface -- List of Contributors -- Part I: Introduction to Regenerative Medicine -- Chapter 1. Current and Future Perspectives of Regenerative Medicine -- REGENERATIVE MEDICINE: CURRENT AND FUTURE PERSPECTIVES -- CONCLUSIONS -- REFERENCES -- Chapter 2. Fundamentals of Cell-Based Therapies -- INTRODUCTION -- RATIONALE FOR CELL-BASED THERAPIES -- CONCLUSIONS -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 3. Stem Cell Research -- INTRODUCTION -- STEM CELLS -- STEM CELL MODULATION IN VITRO -- REGENERATIVE MEDICINE -- CONCLUSION -- ACKNOWLEDGMENTS -- INTERNET RESOURCES -- REFERENCES -- Part II: Biologic and Molecular Basis of Regenerative Medicine -- Chapter 4. Molecular Organization of Cells -- INTRODUCTION -- CELLULAR MECHANISMS OF THE EMT -- MOLECULAR CONTROL OF THE EMT -- CONCLUSION -- REFERENCES -- Chapter 5. Cell-ECM Interactions in Repair and Regeneration -- INTRODUCTION -- COMPOSITION AND DIVERSITY OF THE ECM -- RECEPTORS FOR ECM MOLECULES -- SIGNAL TRANSDUCTION EVENTS DURING CELL-ECM INTERACTIONS -- CELL-ECM INTERACTIONS DURING HEALING OF SKIN WOUNDS -- CELL-ECM INTERACTIONS DURING REGENERATION -- IMPLICATIONS FOR REGENERATIVE MEDICINE -- REFERENCES -- Chapter 6. Developmental Mechanisms of Regeneration -- INTRODUCTION -- MECHANISMS OF REGENERATION -- STRATEGIES OF REGENERATIVE MEDICINE -- CONCLUSION -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 7. The Molecular Basis of Pluripotency in Principles of Regenerative Medicine -- INTRODUCTION TO PLURIPOTENCY -- EXTRACELLULAR SIGNALING FACTORS AND SIGNAL TRANSDUCTION -- TRANSCRIPTIONAL NETWORKS -- EPIGENETIC AND ENVIRONMENTAL REGULATION -- SUMMARY AND PERSPECTIVES -- REFERENCES -- Chapter 8. How Do Cells Change Their Phenotype -- INTRODUCTION -- STEM CELLS -- PLASTICITY - TRANSDIFFERENTIATION AND TRANSDETERMINATION.

CELL FUSION -- CELL PHENOTYPE -- CONTROL OF GENE ACTIVITY -- EXTRINSIC CONTROLS -- CONCLUSIONS -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 9. Somatic Cloning and Epigenetic Reprogramming in Mammals -- INTRODUCTION: SHORT HISTORY OF CLONING -- TECHNICAL ASPECTS OF SOMATIC NT -- SUCCESS RATES OF SOMATIC CLONING AND THE QUESTION OF NORMALITY OF CLONED OFFSPRING -- EPIGENETIC REPROGRAMMING -- APPLICATION OF SOMATIC NT -- CONCLUSIONS -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 10. Transgenic Cloned Goats and Cows for the Production of Therapeutic Proteins -- INTRODUCTION -- GENERATION OF TRANSGENIC ANIMALS -- SCNT: DONOR CELL LINE DEVELOPMENT AND CHARACTERIZATION -- CAPRINE SCNT -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Part III: Cells and Tissue Development -- Chapter 11. Genetic Approaches in Human Embryonic Stem Cells and Their Derivatives -- INTRODUCTION -- MAINTAINING UNDIFFERENTIATED HESCS -- GENETIC APPROACHES TO MANIPULATING HESCS -- DIFFERENTIATION OF HESCS INTO TISSUE-SPECIFIC LINEAGES AND TRANSPLANTATION OF HESC-DERIVED CELLS -- GENETIC MODIFICATIONS OF HESC-DERIVED PROGENIES -- POTENTIAL APPLICATIONS OF GENETICALLY MANIPULATED HESCS AND THEIR DERIVATIVES -- CONCLUSION -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 12. Embryonic Stem Cells: Derivation and Properties -- INTRODUCTION -- DERIVATION OF ES CELLS -- CULTURE OF ES CELLS -- DEVELOPMENTAL POTENTIAL OF ES CELLS -- CONCLUSION -- ACKNOWLEDGMENT -- REFERENCES -- Chapter 13. Stem Cells Derived from Amniotic Fluid and Placenta -- INTRODUCTION -- CVS AND AMNIOCENTESIS -- DIFFERENTIATED CELLS FROM AMNIOTIC FLUID AND PLACENTA -- MESENCHYMAL CELL FROM PLACENTA AND AMNIOTIC FLUID -- PLURIPOTENT STEM CELLS FROM PLACENTA AND AMNIOTIC FLUID -- FUTURE DIRECTION -- SUMMARY -- REFERENCES -- Chapter 14. Stem Cells Derived from Cord Blood -- INTRODUCTION -- PROCUREMENT AND PROCESSING OF CORD BLOOD.

CORD BLOOD STORAGE -- HEMATOPOIETIC AND TISSUE REGENERATION -- PLURIPOTENT CELLS FROM UMBILICAL CORD BLOOD CELLS -- NEUROLOGICAL REGENERATION -- CONCLUSION -- FUTURE DEVELOPMENTS -- REFERENCES -- Chapter 15. Multipotent Adult Progenitor Cells -- PLURIPOTENT STEM CELLS: EMBRYONIC STEM CELLS -- POSTNATAL TISSUE-SPECIFIC STEM CELLS: ARE SOME MORE THAN MULTIPOTENT? -- ISOLATION OF MAPCs -- DIFFERENTIATION ABILITY OF MAPC IN VITRO -- ENGRAFTMENT OF MAPC IN VIVO -- CONTRIBUTION OF MAPC TO CHIMERAS -- MECHANISM UNDERLYING GREATER POTENCY OF MAPC AND SIMILAR ADULT STEM CELLS WITH GREATER POTENCY -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 16. Bone Marrow Stem Cells: Properties and Pluripotency -- INTRODUCTION -- BONE MARROW STEM CELLs -- BMSCS AND TISSUE REGENERATION -- IMPORTANT FACTORS REGULATING BMSC HOMING AND DIFFERENTIATION -- CLINICAL APPLICATIONS OF BMSCS FOR CARDIAC REGENERATION -- CONCLUSION -- REFERENCES -- Chapter 17. Hematopoietic Stem Cell Properties, Markers, and Therapeutics -- INTRODUCTION -- DEVELOPMENTAL ORIGIN OF HEMATOPOIESIS -- INTRINSIC REGULATORS OF SELF-RENEWAL -- MULTI-LINEAGE REPOPULATION -- PLAYERS IN HEMATOPOIESIS -- IN VITRO DIFFERENTIATION OF HEMATOPOIETIC LINEAGES -- IN VITRO EXPANSION OF SELF-RENEWING HSCs -- HSC NICHE -- PURIFICATION AND MOLECULAR SIGNATURE OF HSC -- PLASTICITY AND THERAPEUTICS -- CONCLUSIONS -- REFERENCES -- Chapter 18. Neural Stem Cells -- INTRODUCTION - HISTORICAL BACKGROUND -- THE NEURAL STEM CELL -- ANALYSIS OF NEUROGENESIS -- THERAPEUTICS AND CLINICAL APPROACHES -- CONCLUSION -- REFERENCES -- Chapter 19. Mesenchymal Stem Cells -- INTRODUCTION -- THE DEFINITION OF MSCS -- The Stem Cell Nature of MSCs -- SOURCE OF MSCS AND ISOLATION TECHNIQUES -- WHICH TISSUES CONTAIN MSCS? -- SKELETAL TISSUE REGENERATION BY MSCS -- IMMUNOMODULATORY EFFECTS OF MSCS -- NONSKELETAL TISSUE REGENERATION BY MSCS.

CONCLUSIONS -- REFERENCES -- Chapter 20. Hepatic Stem Cells: Lineage Biology and Pluripotency -- INTRODUCTION -- GENERAL ISSUES WITH RESPECT TO STEM CELLS AND MATURATIONAL LINEAGE BIOLOGY -- SOURCING OF HUMAN TISSUE -- THE LIVER AS A STEM CELL AND MATURATIONAL LINEAGE SYSTEM -- CLINICAL, COMMERCIAL, AND RESEARCH APPLICATIONS OF STEM CELLS (WITH A FOCUS ON LIVER) -- ETHICS AND LEGAL ISSUES IN THE USE OF STEM CELLS -- SUMMARY -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 21. Skeletal Muscle Stem Cells -- INTRODUCTION -- SKELETAL MUSCLE REQUIRES STEM CELL FUNCTION AFTER INJURY OR IN DISEASE -- MUSCLE STEM CELL CRITERIA -- HISTORICAL PERSPECTIVE ON MUSCLE STEM CELL BIOLOGY -- PUTATIVE MUSCLE STEM CELLS AND THEIR DEFINED CHARACTERISTICS -- MUSCLE STEM CELLS IN THEIR NATURAL ENVIRONMENTS IN VIVO -- THE MUSCLE STEM CELL NICHE -- CHALLENGES FACING THE POTENTIAL THERAPEUTIC USE OF MUSCLE STEM CELLS -- CONCLUSION -- REFERENCES -- Chapter 22. Islet Cell Therapy and Pancreatic Stem Cells -- INTRODUCTION -- BENEFITS OF β-CELL REPLACEMENT THERAPY -- CURRENT LIMITATIONS TO β-CELL REPLACEMENT THERAPY -- ALTERNATIVE SOURCES OF INSULIN-PRODUCING CELLS: STEM CELLS AND β-CELL REGENERATION -- PANCREATIC DEVELOPMENT -- ISLET NEOGENESIS FROM ES CELLS -- ISLET NEOGENESIS FROM ADULT STEM CELLS -- TRANSDIFFERENTIATION -- WHAT THE FUTURE MAY HOLD -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 23. Regenerative Medicine for Diseases of the Retina -- INTRODUCTION -- EFTFs: SPECIFICATION OF THE EYE -- RETINAL PROGENITORS: FROM OPTIC CUP TO RETINA -- RETINAL STEM CELLS AND PERSISTENT PROGENITORS IN ADULT VERTEBRATES: THE CILIARY MARGINAL ZONE -- TRANSDIFFERENTIATION AND RETINAL REGENERATION -- CONCLUSION -- REFERENCES -- Chapter 24. Peripheral Blood Stem Cells -- INTRODUCTION -- TYPES AND SOURCE OF STEM CELLS IN THE PERIPHERAL BLOOD -- EPC -- MSC -- HSC.

THERAPEUTIC APPLICATIONS OF PERIPHERAL BLOOD STEM CELLS -- CONCLUSIONS AND FUTURE DIRECTIONS -- REFERENCES -- Chapter 25. Prospects of Somatic Cell Nuclear Transfer-derived Embryonic Stem Cells in Regenerative Medicine -- INTRODUCTION -- BRIEF HISTORY -- STATE OF THE ART -- PROSPECTS AND CHALLENGES -- CONCLUSIONS AND FUTURE DIRECTIONS -- REFERENCES -- Chapter 26. Somatic Cells: Growth and Expansion Potential of T Lymphocytes -- INTRODUCTION -- T CELLS ARE KEY TO IMMUNITY TO INFECTIONS AND CANCER -- GROWTH AND EXPANSION POTENTIAL OF T CELLS -- CELL CULTURE MODELING OF T CELL REPLICATIVE SENESCENCE -- REPLICATIVE SENESCENCE ALSO AFFECTS GENE EXPRESSION AND FUNCTION -- SENESCENT CELLS ARE PRESENT IN VIVO -- SENESCENT CD8 T CELLS ARE ASSOCIATED WITH A VARIETY OF NEGATIVE HEALTH OUTCOMES -- RETARDING OR PREVENTING REPLICATIVE SENESCENCE IN AGING AND HIV DISEASE -- CONCLUDING REMARKS -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 27. Mechanical Determinants of Tissue Development -- INTRODUCTION -- MECHANICAL FORCES EXPERIENCED BY TISSUES -- THE CELL AS A SIGNAL RECEIVER AND PROCESSOR -- OVERVIEW OF BIOREACTORS -- PRACTICAL EXAMPLES OF MECHANICAL DETERMINANTS -- CONCLUSION -- REFERENCES -- Chapter 28. Morphogenesis and Morphogenetic Proteins -- INTRODUCTION -- BMPS -- STEM CELLS -- SCAFFOLDS OF BIOMIMETIC BIOMATERIALS -- CARTILAGE-DERIVED MORPHOGENETIC PROTEINS -- REGENERATIVE MEDICINE AND SURGERY -- ACKNOWLEDGEMENTS -- REFERENCES -- Chapter 29. Physical Stress as a Factor in Tissue Growth and Remodeling -- INTRODUCTION -- STRUCTURAL HIERARCHY AND THE CONTINUUM CONCEPT -- STRAIN AND STRESS DEFINITIONS -- TISSUE GROWTH, REPAIR, AND REMODELING -- MECHANOTRANSDUCTION MECHANISMS -- IN VITRO MECHANICAL CONDITIONING -- CONCLUSIONS -- REFERENCES -- Chapter 30. Engineering Cellular Microenvironments -- INTRODUCTION -- DEFINING THE CELLULAR MICROENVIRONMENT.

DEFINING THE ORGANIZATION OF MULTICELLULAR CONSTRUCTS.