Front cover -- Medicalapplications of Mass Spectrometry -- Copyright page -- Contents -- List of Contributors -- Preface -- Part I: Motivation and Essentials -- Chapter 1. Introduction -- Chapter 2. Basics of analytical chemistry and mass spectrometry for medical professionals -- 1. Introduction -- 2. Terms and definitions -- 3. The analytical procedure -- 4. A case study: analysis of plasma sterol profile -- 5. Mass spectrometry -- Reference -- Chapter 3. Ethical, legal, safety, and scientific aspects of medical research -- 1. Ethical aspects -- 2. Legal aspects -- 3. Safety aspects -- 4. Handling biological materials -- 5. Clinical trials and protocols -- 6. Administrative procedures -- References -- Part II: Tools of the Trade -- Chapter 4. Biomedical sampling -- 1. Sampling -- 2. Sample preparation -- 3. Extraction techniques -- 4. Automation and high throughput -- 5. Outlook -- References -- Chapter 5. Separation methods -- 1. Introduction -- 2. Gas chromatography -- 3. High-performance liquid chromatography -- 4. Electrophoretic techniques -- 5. Future trends -- References -- Chapter 6. Mass spectrometry instrumentation and techniques -- 1. Introduction -- 2. General questions about mass measurement and mass spectrometry -- 3. Separation techniques: gas chromatography (GC), and high-performance liquid chromatography (HPLC) -- 4. Ionization methods -- 5. Mass analyzers -- 6. Tandem mass spectrometry (MS/MS) -- 7. Selected terms for clarification -- Acknowledgment -- References -- Chapter 7. Chemoinformatics-multivariate mathematical-statistical methods for data evaluation -- 1. Introduction -- 2. Data types and data pretreatment -- 3. Multivariate methods -- 4. Selected applications of chemometrics -- Terms and terminology -- References -- Part III: Biomolecules -- Chapter 8. Mass spectrometry in proteomics -- 1. Introduction.

2. Methods in proteomics -- 3. Outlook -- References -- Chapter 9. De novo sequencing of peptides -- Chapter 10. Protein bioinformatics -- 1. Highlights for medical professionals -- 2. Introduction -- 3. Methodology -- 4. Discussion -- 5. Future trends -- 6. Conclusions -- Acknowledgments -- References -- Chapter 11. Analysis of complex lipidomes -- 1. Introduction -- 2. Methodology -- 3. Phospholipids -- 4. Acylglycerols -- 5. Sphingolipids -- 6. Sterols -- 7. Medical applications of MS-lipidomics -- 8. Future trends -- 9. Conclusions -- References -- Part IV: Selected Medical Applications -- Chapter 12. Medical laboratory practice-possibilities for mass spectrometry -- 1. Introduction -- 2. Quality management -- 3. Inborn errors of metabolism -- 4. Assessment of in vivo metabolism using stable isotope techniques -- 5. Conclusions -- References -- Chapter 13. Therapeutic drug monitoring and measurement of drug concentrations using mass spectrometry -- 1. Introduction -- 2. Antiinfection drugs -- 3. Drugs acting on the central nervous system (CNS) -- 4. Cardiovascular drugs -- 5. Anticancer agents -- 6. Analgesics -- 7. Miscellaneous drug classes -- References -- Chapter 14. Mass spectrometry of infectious pathogens -- 1. Introduction -- 2. Methodology -- 3. Future trends and prospects -- References -- Chapter 15. Proteomics of viruses -- 1. Introduction -- 2. Virus-host interactions -- 3. Diagnostics -- 4. Discussion -- 5. Future trends -- 6. Conclusions -- Acknowledgments -- References -- Chapter 16. Neonatal research -- 1. Introduction -- 2. Principle of the methodology -- 3. Quick reference for practical implementation of the methodology -- 4. Key points of the methodology -- 5. Ongoing extensions of NBS -- 6. Conclusions -- Acknowledgments -- References -- Chapter 17. Applications of mass spectrometry in oncology -- 1. Introduction.

2. New methodology-SELDI-TOF-MS -- 3. Other relevant methodological challenges -- 4. Diagnostic oncoproteomics based on SELDI-TOF-MS -- 5. Representative other applications -- References -- Chapter 18. Application of mass spectrometry in brain research: neurotransmitters, neuropeptides, and brain proteins -- 1. Introduction -- 2. Methodology -- 3. Discussion -- 4. Future trends -- 5. Conclusions -- Acknowledgments -- References -- Chapter 19. The human pituitary proteome: clinical applications -- 1. Introduction -- 2. The pituitary gland and mass spectrometry: an endocrinologist's perspective -- 3. Methodology -- 4. Discussion -- 5. Future trends -- 6. Conclusions -- Acknowledgments -- References -- Chapter 20. Mass spectrometry of proteinous allergens inducing human diseases -- 1. Introduction -- 2. Methodology -- 3. Mass spectrometric identification of proteinous allergens -- 4. Future trends -- 5. Conclusions -- References -- Chapter 21. Mass spectrometry in clinical treatment -- 1. Introduction -- 2. Pediatrics -- 3. Oncology -- References -- Part V: Emerging Areas -- Chapter 22. Biomarker discovery -- 1. Introduction -- 2. Biomarkers in medicine -- 3. Important definitions -- 4. Biomarkers discovery and complexity of biological systems -- 5. Biomarker discovery and "omics" -- 6. The biomarker discovery project -- 7. Challenges in the biomarker discovery pathway -- 8. Technical issues in experimental design -- 9. The discovery process overview -- 10. Conclusions -- References -- Chapter 23. Molecular imaging by mass spectrometry -- 1. Introduction -- 2. Methods -- 3. Discussion -- 4. Future trends -- 5. Conclusion -- Acknowledgments -- References -- Chapter 24. Brief outlook -- 1. Trends in instrumentation -- 2. Emerging systems approach -- 3. Mass spectrometry in translational medicine -- Index.