Contents -- Contributors -- Preface -- Glossary of Terms -- A -- C -- E -- G -- H -- I -- K -- L -- M -- O -- P -- R -- S -- T -- PART I: IDENTIFICATION AND DIAGNOSTICS -- 1 Molecular Approaches to Virus Characterization and Detection -- 1.1. Introduction -- 1.2. Current Virus Taxonomy and Classification -- 1.3. Preliminary Approaches to Virus Identification -- 1.4. Methodology for Virus Isolation and Fractionation (Nucleic Acid and Protein Purification Techniques) -- 1.5. Biochemical/Molecular Approaches to Virus Identification and Diagnosis -- 1.6. PCR and Virus-specific Primer Development -- 1.7. Conclusions -- 2 Molecular Approaches and Techniques for the Study of Entomopathogenic Bacteria -- 2.1. Introduction -- 2.2. Classification of Entomopathogenic Bacteria -- 2.3. Conclusions -- 3 Molecular Methods for Identification and Diagnosis of Fungi -- 3.1. Introduction -- 3.2. General Considerations -- 3.3. Genetic Fingerprinting -- 3.4. DNA Sequencing -- 3.5. Diagnosis and Detection -- 3.6. Conclusions and Future Prospects -- 4 Molecular Approaches and the Taxonomy of Insect-parasitic and Pathogenic Nematodes -- 4.1. Introduction -- 4.2. Nematode Diagnosis and the Barcode System -- 4.3. A Review of Molecular Approaches Considered for Insect-parasitic and Pathogenic Nematode Taxonomy -- 4.4. Techniques Considered for Obtaining DNA Sequences -- 4.5. Sequencing -- 4.6. Sequence Manipulation and Analysis -- 4.7. Conclusions -- 5 Identification and Diagnostics of Entomopathogenic Protozoa -- 5.1. Introduction -- 5.2. Molecular Identification of Species and Strains -- 5.3. Conclusion and Future Perspectives -- PART II: EVOLUTIONARY RELATIONSHIPS AND POPULATION GENETICS -- 6 Phylogenetic Studies with Entomopathogenic Bacteria with Special Emphasis on Symbionts of Entomopathogenic Nematodes -- 6.1. Introduction -- 6.2. Genes Considered.

6.3. Conclusions and Perspectives -- 7 Molecular Systematics of Entomopathogenic Fungi -- 7.1. Introduction -- 7.2. Molecular Phylogenies of Fungi and the Origins of Entomopathogens -- 7.3. Species Recognition -- 7.4. Species-level Phylogenies of Entomopathogenic Fungi -- 7.5. Conclusions -- 8 Phylogenetics and Population Genetics of Entomopathogenic and Insect-parasitic Nematodes -- 8.1. Introduction -- 8.2. Phylogenetics -- 8.3. Population Genetics -- 8.4. DNA Bar Coding -- 8.5. DNA Markers Considered for Phylogenetic and Population Genetics Studies -- 8.6. Methodology -- 8.7. Co-phylogenesis and Cospeciation -- 8.8. Population Genetics Methods -- PART III: HOST-PATHOGEN INTERACTIONS -- 9 Host-Virus Interactions -- 9.1. Introduction -- 9.2. Use of Viruses as Insect Pest Control Agents -- 9.3. Development of Baculoviruses for Foreign Gene Expression -- 9.4. Insect Defences Against Viruses -- 9.5. Baculovirus Pathogenesis -- 9.6. Baculovirus Host Range -- 9.7. Unclassified DNA Viruses -- 9.8. Mechanisms of Insect Virus Persistence -- 9.9. Conclusions and Future Prospects -- 10 Insect-Protozoa-Bacteria Associations: a Model System for Investigating Host-Parasite Interactions -- 10.1. Introduction -- 10.2. Trypanosomatid Protozoa and Tsetse Flies -- 10.3. Molecular Approaches and Their Application to Study Insect Host Immune Responses -- 10.4. Tsetse Endosymbionts -- 10.5. Control of Insect-borne Diseases -- 10.6. Current and Future Work -- 10.7. Conclusions -- 11 Methods in Investigating Nematode-Bacterium-Insect Symbiosis -- 11.1. Introduction -- 11.2. Molecular Tools to Study Entomopathogenic Nematodes -- 11.3. Basic Molecular Tools for the Study of Entomopathogenic Bacteria -- 11.4. Techniques to Investigate Bacteria-Nematode Mutualism -- 11.5. Techniques in Studying EPB Virulence -- 11.6. Conclusions and Future Prospects.

PART IV: GENOMICS AND GENETIC ENGINEERING -- 12 Genetic Engineering of Bacteria to Improve Efficacy Using the Insecticidal Proteins of Bacillus Species -- 12.1. Introduction -- 12.2. Basic Biology of Bacillus thuringiensis -- 12.3. Insecticidal Proteins of Bacillus thuringiensis -- 12.4. Genetic Factors Regulating Insecticidal Proteins -- 12.5. Construction of Recombinant Bacteria -- 12.6. Conclusions and Future Prospects -- 13 Genomic Analysis of the Symbiotic and Entomopathogenic Photorhabdus Bacteria -- 13.1. Introduction -- 13.2. Sequencing and Annotation of Photorhabdus Genomes -- 13.3. Main Features of the P. luminescens Genome -- 13.4. Analogical Post-genomic Analysis -- 13.5. Post-genomic Analysis by a 'Blind' Approach -- 13.6. Conclusions and Future Perspectives -- 14 Genomics of Entomopathogenic Viruses -- 14.1. Introduction -- 14.2. General Concepts -- 14.3. Analyses of DNA and Protein Sequences -- 14.4. Genetic Modification of Baculoviruses -- 14.5. Conclusions -- 15 Genomics and Genetic Improvement of Entomopathogenic Nematodes -- 15.1. Introduction -- 15.2. Genomic Sequencing and Bioinformatics -- 15.3. Functional Genomics: Towards Deciphering of Genomics and ESTs Sequences -- 15.4. Genetic Improvement -- 15.5. Conclusion and Future Prospects -- 16 Entomopathonic Fungi and the Genomics Era -- 16.1. Introduction -- 16.2. Procedures for Isolating Pathogen DNA -- 16.3. Procedures for Isolating Pathogen RNA -- 16.4. Pulsed Field Gel Electrophoresis -- 16.5. Construction of Cloning and Expression Vector Components and Markers -- 16.6. Transformation Systems -- 16.7. Gene Cloning Strategies -- 16.8. Analysing Differential Gene Expression -- 16.9. EST Screening -- 16.10. Microarray Analysis -- 16.11. Targeted Gene Mutagenesis -- 16.12. RNA Interference -- 16.13. Conclusions -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L.

M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- X -- Y -- Z.