ROUTE MAPS IN GENE TECHNOLOGY -- CONTENTS -- PREFACE -- ABOUT THE ROUTE MAPS FORMAT -- 1. THE CONCEPT OF GENES IS DEVELOPED -- 2. GENES ARE LOCATED TO CHROMOSOMES -- 3. GENES ARE COMPOSED OF DNA -- 4. THE CHEMICAL BUILDING BLOCKS OF NUCLEIC ACIDS -- 5. FORMATION OF THE DNA DOUBLE HELIX -- 6. PACKAGING OF DNA WITHIN CELLS -- 7. CHROMATIN STRUCTURE AND THE FUNCTIONAL ACTIVITY OF GENES -- 8. TYPES AND FUNCTIONS OF DNA-PROTEIN INTERACTIONS -- 9. ORGANISATION OF GENOMES INTO MULTIPLE CHROMOSOMES -- 10. DISTRIBUTION OF NUCLEIC ACIDS WITHIN EUKARYOTIC CELLS -- 11. TYPES OF RNA MOLECULES -- 12. THE ANATOMY OF EUKARYOTIC CHROMOSOMES -- 13. ORGANISATION OF GENES WITHIN CHROMOSOMES -- 14. THE MOLECULAR ANATOMY OF EUKARYOTIC GENES -- 15. CHROMOSOME ABERRATIONS AND LINKS TO HUMAN DISEASE -- 16. TYPES OF MUTATIONS AND THEIR EFFECTS -- 17. FORMS OF CHEMICALLY ALTERED DNA -- 18. DNA REPAIR MECHANISMS -- 19. LINKAGE ANALYSIS -- 20. PEDIGREE ANALYSIS AND MODES OF INHERITANCE -- 21. GENES DICTATE THE NATURE OF PROTEINS -- 22. THE NATURE OF THE GENETIC CODE -- 23. TRANSCRIPTION: FORMING GENETIC MESSAGES -- 24. POST-TRANSCRIPTIONAL PROCESSING OF MESSENGER RNA -- 25. TRANSFER AND RIBOSOMAL RNA PROCESSING/MODIFICATION -- 26. MECHANISMS REGULATING GENE EXPRESSION -- 27. TRANSCRIPTIONAL REGULATORY SEQUENCES -- 28. OPERONS AND PROKARYOTIC CONTROL OF GENE EXPRESSION -- 29. TRANSCRIPTION FACTORS AND GENE EXPRESSION -- 30. IN VIVO TRANSLATION: DECODING GENETIC MESSAGES -- 31. SEQUENCES INVOLVED IN CELLULAR PROTEIN TARGETING -- 32. EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS -- 33. MOLECULAR MECHANISMS OF CELL CYCLE CONTR0L -- 34. GENETIC RECOMBINATION MECHANISMS -- 35. GENE TRANSFER DURING BACTERIAL REPRODUCTION -- 36. TRANSPOSABLE GENETIC ELEMENTS: TRANSPOSONS -- 37. IN VIVO DNA REPLICATION -- 38. GENETIC CONTROL OF DEVELOPMENT -- 39. THE NATURAL BIOLOGY OF BACTERIOPHAGES.

40. BACTERIOPHAGE GENETICS -- 41. RECOMBINANT DNA TECHNOLOGY -- 42. ENZYMES COMMONLY USED IN MOLECULAR BIOLOGY METHODS -- 43. RESTRICTION ENDONUCLEASES -- 44. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS -- 45. ISOLATION OF NUCLEIC ACIDS FROM CELLS AND TISSUES -- 46. VISUALISING NUCLEIC ACIDS -- 47. ELECTROPHORESIS OF NUCLEIC ACIDS -- 48. IN VITRO HYBRIDISATION -- 49. TYPES OF HYBRIDISATION ASSAY FORMATS -- 50. SOUTHERN BLOTTING -- 51. IN SITU HYBRIDISATION -- 52. MEASURING TRANSCRIPTIONAL ACTIVITY VIA MESSENGER RNA -- 53. CONVERTING MESSENGER RNA INTO COMPLEMENTARY DNA -- 54. METHODS FOR DETERMINING DNA NUCLEOTIDE SEQUENCES -- 55. THE POLYMERASE CHAIN REACTION -- 56. ALTERNATIVES TO PCR-BASED IN VITRO DNA/RNA AMPLIFICATION -- 57. IN VITRO TRANSLATION METHODS -- 58. TYPES AND METHODS OF GENE PROBE GENERATION -- 59. CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES -- 60. TYPES AND APPLICATIONS OF NUCLEOTIDE ANALOGUES -- 61. METHODS FOR LABELLING GENE PROBES -- 62. FUNDAMENTAL PRINCIPLES OF CLONING -- 63. THE NATURE OF CLONING VECTORS -- 64. INSERTING FOREIGN DNA INTO VECTORS -- 65. THE DEVELOPMENT OF BACTERIOPHAGE VECTORS -- 66. PLASMIDS: DEVELOPMENT AS CLONING VECTORS -- 67. YEAST-DERIVED PLASMID VECTORS -- 68. PHAGEMIDS, HYBRID PHAGE AND PLASMID CLONING VECTORS -- 69. VECTORS FOR USE IN PLANT AND ANIMAL CELLS -- 70. DELIVERING DNA INTO CELLS -- 71. PRINCIPAL GENOMIC AND cDNA CLONING STRATEGIES -- 72. STRATEGIES FOR IDENTIFYING DESIRABLE RECOMBINANT CLONES -- 73. GENE MAPPING TECHNIQUES -- 74. DETECTING DNA-PROTEIN INTERACTION SITES -- 75. DETECTING PROMOTER AND ENHANCER SEQUENGES -- 76. METHODS FOR IDENTIFYING PROTEIN-ENCODING REGIONS -- 77. GENETIC FINGERPRINTING -- 78. ANALYSING ANCIENT DNA -- 79. IN VITRO MUTAGENESIS METHODS -- 80. GENETICALLY MODIFIED MICRO-ORGANISMS -- 81. GENETICALLY ENGINEERED PLANTS -- 82. GENETICALLY ENGINEERED ANIMALS.

83. MOLECULAR TECHNIQUES IN PRENATAL DIAGNOSIS -- 84. THE GENETICS OF CYSTIC FIBROSIS -- 85. THE DYSTROPHIN GENE AND MUSCULAR DYSTROPHIES -- 86. IDENTIFYING THE GENE FOR HUNTINGTON'S DISEASE -- 87. LIPOPROTEIN GENES AND CORONARY HEART DISEASE -- 88. THE DETECTION OF MICROBIAL INFECTIONS -- 89. MOLECULAR BIOLOGY OF HUMAN IMMUNODEFICIENCY VIRUS AND AIDS -- 90. ENGINEERING MICROBIAL BIOLUMINESCENCE -- 91. RECOMBINANT DNA METHODS IN VACCINE DEVELOPMENT -- 92. RECEPTORS AND CELLULAR SIGNALLING MECHANISMS -- 93. ONCOGENES AND THE MOLECULAR BASIS OF CANCER -- 94. MOLECULAR DIAGNOSIS AND THERAPY OF CANCERS -- 95. DRUG DEVELOPMENT USING RECOMBINANT DNA APPROACHES -- 96. PROTEIN ENGINEERING -- 97. IMMUNOGLOBULIN GENETICS -- 98. GENETIC ENGINEERING OF RECOMBINANT ANTIBODIES -- 99. CURRENT APPROACHES TO GENE THERAPY -- 100. THE HUMAN GENOME MAPPING PROJECT -- BIBLIOGRAPHY -- 1: ORIGINAL ARTICLES -- 2: REVIEW ARTICLES -- 3: SUGGESTED FURTHER READING -- INDEX.