CONTENTS -- Preface -- Organizing Committee -- Contributor Contact Details -- PART I. General -- 1. Biomaterials and Mechanical Stimulation in Tissue Engineering Tetsuya Tateishi, Guoping Chen, Naoki Kawazoe, Makoto Kawanishi, and Takashi Ushida -- 1 Introduction -- 2 Biomaterials for Scaffold [1-9] -- 3 Biodegradable Polymer Composite Sponge -- 4 Biodegradable Polymer Composite Sponge -- 5 Composite Sponge of Synthetic Polymer, Collagen and Hydroxyapatite -- 6 Tissue Engineered Articular Cartilage -- 7 Gas-controlled Cyclic Hydrostatic Pressure System for Bioreactor -- 8 Conclusions -- Acknowledgments -- References -- 2. Osteoinduction and Ostogenic Genes Expression Regulated by Ca-P Bioceramics Xingdong Zhang -- 1 Introduction -- 2 Summary -- References -- 3. Why is the Clinical Application of Tissue Engineering So Slow? Yoshito Ikada -- 1 Introduction -- 2 Surgery Before Tissue Engineering -- 3 Emergence of Tissue Engineering -- 4 Principle of the Current Tissue Engineering -- 5 Reasons for Slow Clinical Application of Tissue Engineering -- 5.1 Cells -- 5.2 Scaffold -- 5.3 Growth Factors -- 6 Ex Vivo and In Situ Tissue Engineering -- 7 Clinical Trials of Tissue Engineering -- 7.1 Skin -- 7.2 Articular Cartilage -- 7.3 Bone -- 7.4 Mandible -- 7.5 Orbital Floor -- 7.6 Vascular Tissue -- 7.7 Pericardial Tissue -- 7.8 Dura Mater -- 8 Conclusions -- References -- 4. Temperature-responsive Cell Culture Surfaces for Cell Sheet Tissue Engineering Zhonglan Tang, Yoshikatsu Akiyama, and Teruo Okano -- 1 Introduction -- 2 Temperature-Responsive Cell Culture Surfaces for Reversible Cellular Adhesion and Detachment -- 2.1 Ultrathin Poly(N-isopropylacrylamide) Gel Grafted on Cell Culture Surfaces -- 2.2 Cell Sheet Engineering -- 3 Functionalization of Temperature-Responsive Cell Culture Surfaces.

3.1 Incorporation of Poly(n-butyl Methacrylate) to Intelligent Surfaces -- 3.2 Co-grafting of Poly(N-isopropylacrylamide) and Poly(n-butyl Methacrylate) to Intelligent Surfaces -- 3.3 Biofunctionalized Intelligent Surfaces with 2-Carboxy-isopropyl- Acrylamide -- 4 Rapid Recovery of Cell Sheets -- 4.1 Poly(N-isoproplacrylamide) Grafted on Porous Cell Culture Membranes -- 4.2 Poly(Ethylene Glycol ) Co-grafted with Poly(N-Isoproplacrylamide) onto Porous Cell Culture Membranes -- 5 Conclusion -- References -- PART II. Tissue Engineering -- 5. Adipose Tissue-derived Mesenchymal Stem Cells have Lower Osteogenic Potential than Bone Marrow-derived Mesenchymal Stem Cells Yoshihiro Katsube, Ousuke Hayashi, Motohiro Hirose, and Hajime Ohgushi -- 1 Introduction -- 2 Isolation and Culture of Stem Cells -- 3 Colony-Forming Capabilities of MSCs -- 4 Osteogenic Differentiation of MSCs -- 4.1 In Vitro Analysis -- 4.2 In Vivo Analysis -- 5 Conclusions -- Acknowledgments -- References -- 6. Amelogenin Overexpression in Tooth Development Akiyoshi Taniguchi and Liming Xu -- 1 Introduction -- 2 The Regulation Mechanisms of Amelogenin mRNA -- 3 The Molecular Mechanism of Enhanced Amelogenin mRNA Stabilization -- 4 The Effects of LAMP1 and LAMP3 on M180 Amelogenin Uptake, Localization and Amelogenin mRNA Induction by Amelogenin Protein -- 5 Discussions -- References -- 7. Mechano-active Tissue Engineering Sang-Heon Kim, Youngmee Jung, Soo Hyun Kim, and Young Ha Kim -- 1 Introduction -- 2 Mechano-biology -- 3 Mechano-active Scaffolds -- 4 Mechano-active Vascular Tissue Engineering -- 5 Mechano-active Cartilage Tissue Engineering -- 6 Conclusions -- Acknowledgments -- References -- 8. Biomaterials for In Vitro Expansion of Stem Cells Yoshihiro Ito -- 1 Introduction -- 2 Culture of Human Umbilical Cord Blood Cells.

2.1 Effect of Fixation Methods of Stromal Cells on the Ex Vivo Expansion of Human CB Progenitors -- 2.2 Stromal Cell Contact Promoted the Expansion of Progenitors -- 2.3 GA-fixed Stromal Cells Stored for Several Weeks Maintained the Ability to Expand Human CB Progenitors -- 3 Culture of ES Cells -- 3.1 Chemically Fixed Cells for ES Cells -- 3.2 Synthetic Biomateirals for ES Cells -- 4 Conclusion -- Acknowledgments -- References -- PART III. Polymeric Biomaterials -- 9. Design of Artificial Extracellular Matrices and Their Application for Regenerative Medicine Xiaoshan Yue, Masato Nagaoka, and Akaike Toshihiro -- 1 Introduction -- 2 Extracellular Matrices for Regenerative Medicine -- 2.1 Regenerative Medicine by Using Stem Cells -- 2.2 Extracellular Matrices for Controlling ES Cell Differentiation -- 2.3 Regulation of ES Cells Differentiation by Other Signal Induction -- 2.4 Application of Artificial Materials to Regenerative Medicine -- 3 Design and Application of Engineered Matrices by Genetic Technology -- 3.1 Design and Application of Fusion Protein for the Regulation of Cell Function -- 3.2 Application of Artificial Extracellular Matrices to ES Cell Culture -- 3.3 Application of Artificial Extracellular Matrices for Controlling Cell Functions -- 4 Conclusions -- Acknowledgments -- References -- 10. Nano-to-macro Architectures Polycaprolactone-based Biomaterials in Tissue Engineering Teoh Swee Hin, Bina Rai, Tiaw Kay Siang, Chong Seow Khoon Mark, Zhang Zhiyong, and Teo Yiling Erin -- 1 Introduction -- 2 Development of "Cell/PCL Film" Sheet Technology -- 3 PCL Films with Laser-Modified Surfaces -- 4 PCL Scaffolds for Drug Delivery Applications -- 5 PCL-TCP Scaffolds for Growth Factor-based BTE -- 6 PCL-TCP Scaffolds for Cell-based BTE -- 7 Conclusions -- References.

11. Nanofiber-based Scaffolds for Tissue Engineering Hisatoshi Kobayashi, Yoshiro Yokoyama, Chiaki Yoshikawa, Satoshi Igarashi, Shinya Hattori, Takako Honda, Hiroyuki Koyama, and Tsuyoshi Takato -- 1 Introduction -- 2 Nanofiber Construct Preparation by Electrospinning -- 2.1 Micro-structured Nanofiber Mat with Template Collector System -- 2.2 Sponge Form Preparation with Wet Bath Collector System -- 3 In Vitro and In Vivo Evaluation for the PGA Nanofiber Fabrics -- 3.1 In Vitro Evaluation of the PGA Spongiform Nanofiber Fabric -- 3.2 In Vivo Evaluation of the PGA Spongiform Nanofiber Fabric -- 4 Conclusions -- Acknowledgments -- References -- 12. Electrospun Composite Nanofibrous Scaffolds for Tissue Engineering Inn-Kyu Kang and Jung Chul Kim -- Abstract -- 1 Introduction -- 2 Materials and Methods -- 2.1 Electrospinning & Surface Characterization -- 2.2 Biodegradation & Cell Behaviors -- 2.3 Open Wound Healing Test -- 3 Results -- 3.1 Morphology of Electrospun Nanofibrous Scaffolds -- 3.2 Characterization of Nanofibrous Scaffolds -- 3.3 In Vitro Biodegradation -- 3.4 Cell-scaffold Interaction -- 4 Discussion -- References -- 13. Synthetic/Natural Hybrid Scaffold for Cartilage and Disc Regenerations Gilson Khang, Soon Hee Kim, John M. Rhee, Munirah Sha'Ban, and Ruszymah Bt Hj Idrus -- 1 Introduction -- 2 Biomaterials for Tissue Engineering -- 2.1 Importance of Scaffold Matrices in Tissue Engineering -- 2.2 Natural Polymers -- 2.3 Synthetic Polymers and Poly( α-hydroxy ester)s -- 2.4 Bioceramic Scaffolds -- 3 Natural/synthetic Hybrid Scaffolds for Tissue Engineering -- 3.1 PLGA Hybrid Scaffolds -- 3.2 Fibrin/PLGA Hybrid Scaffolds for Cartilage Regeneration In Vivo and In Vitro -- 3.3 Fibrin/PLGA Hybrid Scaffolds for IVD In Vitro -- 4 Conclusions -- Acknowledgments -- References.

14. Novel Hydrogel Systems as Injectable Scaffolds for Tissue Engineering Yoon Ki Joung and Ki Dong Park -- 1 Introduction -- 2 Chitosan-Pluronic Hydrogels -- 3 Tetronic-PLA-Heparin Hydrogels -- 4 Heparin-Containing PLGA-PEG-PLGA Hydrogels -- 5 Four-Arm PEG-PLA Stereo-Complex Systems -- 6 Conclusions -- Acknowledgments -- References -- 15. Manipulation of Stem Cell Functions on Grafted Polymer Surfaces Naoki Kawazoe, Likun Guo, Guoping Chen, and Tetsuya Tateishi -- 1 Introduction -- 2 Chondrogenic Differentiation of Human Mesenchymal Stem Cells on Polymer-Grafted Surfaces -- 2.1 Surface Grafting of Polyallylamine, Poly(acrylic acid ), and Poly(ethylene glycol ) -- 2.2 Cell Adhesion, Proliferation, and Differentiation -- 2.2.1 Cell adhesion and aggregation -- 2.2.2 Cell proliferation -- 2.3 Chondrogenic Differentiation -- 2.3.1 Histological staining -- 2.3.2 Immunohistological staining -- 2.3.3 Gene expression -- 2.4 The Effects of the Polymer Charges on Chondrogenesis -- 3 Adipogenic Differentiation of Mesenchymal Stem Cells on Micropatterned Polymer Surfaces -- 3.1 Preparation of Micropatterned Polymer Surfaces -- 3.2 Cell Adhesion on Micropatterned Polymer Surfaces -- 3.2.1 Cell culture -- 3.2.2 Cell adhesion and proliferation -- 3.3 Adipogenic Differentiation on Micropatterned Polymer Surfaces -- 3.3.1 Formation of lipid vacuoles -- 3.2.2 Gene expression analysis -- 3.4 The Effects of the Grafted Polymer on Adipogenesis -- 4 Conclusion -- Acknowledgments -- References -- 16. Gamma-ray Irradiated Poly(L-lactide) for Bone Repair Kazuo Isama and Toshie Tsuchiya -- 1 Introduction -- 2 Wear Characteristic of the Gamma-ray Irradiated PLLA Sheets -- 2.1 Gamma-ray Irradiation of the PLLA Sheets -- 2.2 Molecular Weight of the PLLA -- 2.3 Wear Test -- 2.4 Coulter Counter Analysis of Wear Debris.

2.5 Particle Size Distribution of Wear Debris from the PLLA Sheets.