Contents -- Editorial Board -- Contributors -- Preface -- SECTION A: GENERAL METHODS -- 1 Proteomics and Genomics Methodology in the Clinical Immunology Laboratory -- 2 Introduction to Protein Analysis -- 3 Protein Analysis for Diagnostic Applications -- 4 Genomics and Chip Technology -- 5 Introduction to Molecular Methodology -- SECTION B: IMMUNOGLOBULIN METHODS -- 6 Introduction -- 7 Immunoglobulin Genes -- 8 Immunoglobulin Quantification and Viscosity Measurement -- 9 Clinical Indications and Applications of Serum and Urine Protein Electrophoresis -- 10 Immunochemical Characterization of Immunoglobulins in Serum, Urine, and Cerebrospinal Fluid -- 11 Cryoglobulins, Cryofibrinogenemia, and Pyroglobulins -- SECTION C: COMPLEMENT -- 12 Analysis of Complement in the Clinical Laboratory -- 13 Molecular and Clinical Evaluation of Atypical Hemolytic Uremic Syndrome -- 14 An Enzyme-Linked Immunosorbent Assay-Based Method for Functional Analysis of the Three Pathways of the Complement System -- 15 Evaluation of the Mannan-Binding Lectin Pathway of Complement Activation -- 16 Complement in Transplant Rejection -- SECTION D: FLOW CYTOMETRY -- 17 Introduction -- 18 Flow Cytometry-Based Immunophenotyping Method and Applications -- 19 Nontraditional Clinical Applications: Paroxysmal Nocturnal Hemoglobinuria Testing, Fetal Red Cell Testing, T-Cell Receptor Vβ Analysis, and Platelet Analysis by Flow Cytometry -- 20 Immunophenotyping of Leukemia and Lymphoma by Flow Cytometry -- 21 Standardized Flow Cytometry Assays for Enumerating Hematopoietic Stem Cells -- 22 Phenotypic Correlates of Genetic Abnormalities in Acute and Chronic Leukemias -- 23 Multimeric Major Histocompatibility Complex Reagents for the Detection and Quantitation of Specific T-Cell Populations.

24 In Situ Gene Amplification and Hybridization Assays: Disease Monitoring Applications Using Flow Cytometry -- SECTION E: FUNCTIONAL CELLULAR ASSAYS -- 25 Introduction -- 26 Delayed-Type Hypersensitivity Skin Testing -- 27 Cryopreservation of Peripheral Blood Mononuclear Cells -- 28 Methods for Detection of Antigen-Specific T Cells by Enzyme-Linked Immunospot Assay (ELISPOT) -- 29 Evaluation of the T-Cell Receptor Repertoire -- 30 Molecular Diagnosis of Chronic Granulomatous Disease -- 31 Clinical Evaluation of Myeloid and Monocytic Cell Functions -- 32 Flow Cytometric Detection and Quantification of Apoptotic Cells -- 33 Quantification of Recent Thymic Emigrants: T-Cell-Receptor Excision Circles -- 34 Measurement of NK-Cell Activity in Humans -- 35 Cellular Immune Assays for Evaluation of Vaccine Efficacy -- 36 T-Lymphocyte Activation and Cell Signaling -- 37 Functional Assays for B Cells and Antibodies -- SECTION F: CYTOKINES AND CHEMOKINES -- 38 Introduction -- 39 Multiplex Cytokine Assays -- 40 Monitoring of Immune Response Using Cytokine Flow Cytometry -- 41 Molecular Analysis of Cytokines and Cytokine Receptors -- 42 Chemokine and Chemokine Receptor Analysis -- 43 Laboratory Monitoring of Cytokine Therapy -- SECTION G: IMMUNOHISTOLOGY AND IMMUNOPATHOLOGY -- 44 Introduction -- 45 Immunohistochemistry: Principles and Advances -- 46 Use of Molecular Biology Methods on Tissues -- 47 Immunohistochemistry: Diagnostic and Prognostic Applications -- 48 Immunofluorescence Methods in the Diagnosis of Renal and Skin Diseases -- SECTION H: INFECTIOUS DISEASES CAUSED BY BACTERIA, MYCOPLASMAS, CHLAMYDIAE, AND RICKETTSIAE -- 49 Introduction -- 50 Diagnostic Methods for Group A Streptococcal Infections -- 51 Immune Responses to Polysaccharide and Conjugate Vaccines.

52 Corynebacterium diphtheriae and Clostridium tetani: Immune Response and Diagnostic Methods -- 53 Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic Enterobacteriaceae and Vibrionaceae -- 54 Serologic and Molecular Diagnosis of Helicobacter pylori Infection and Eradication -- 55 Detection of Antibodies to Legionella -- 56 Immunologic Methods for Diagnosis of Spirochetal Diseases -- 57 Lyme Disease: Serologic Assays for Antibodies to Borrelia burgdorferi -- 58 Immunological Tests in Tuberculosis and Leprosy -- 59 Mycoplasma: Immunologic and Molecular Diagnostic Methods -- 60 Chlamydial Infections -- 61 The Rickettsiaceae, Anaplasmataceae, Bartonellaceae, and Coxiellaceae -- 62 Serologic and Molecular Tools for Diagnosing Bordetella pertussis Infection -- 63 Bacillus anthracis Detection and Anthrax Diagnosis -- SECTION I: MYCOTIC AND PARASITIC DISEASES -- 64 Introduction -- 65 Molecular and Immunological Approaches to the Diagnosis of Parasitic Infections -- 66 Serological and Molecular Diagnosis of Fungal Infections -- SECTION J: VIRAL DISEASES -- 67 Introduction -- 68 Rapid Viral Diagnosis -- 69 Enhanced Detection of Viruses in Cell Cultures -- 70 Herpes Simplex Virus -- 71 Varicella-Zoster Virus -- 72 Epstein-Barr Virus -- 73 Cytomegalovirus -- 74 Human Herpesviruses 6, 7, and 8 -- 75 Papillomaviruses and Polyomaviruses -- 76 Adenoviruses -- 77 Parvovirus B19 -- 78 Influenza Viruses -- 79 Respiratory Syncytial Virus, Human Metapneumovirus, and the Parainfluenza Viruses -- 80 Measles and Mumps -- 81 Rubella Virus -- 82 Enteroviruses -- 83 Viral Hepatitis -- 84 Rotaviruses and Noroviruses -- 85 Arboviruses -- 86 Hantavirus Infections -- 87 Filoviruses and Arenaviruses -- 88 Rabies Virus -- 89 Human T-Cell Lymphotropic Virus Types 1 and 2 -- 90 Coronavirus Including Severe Acute Respiratory Syndrome (SARS) -- 91 Poxviruses.

92 Prion Diagnostics -- SECTION K: HUMAN IMMUNODEFICIENCY VIRUS -- 93 Introduction -- 94 Principles and Procedures of Human Immunodeficiency Virus Serodiagnosis -- 95 General Immunologic Evaluation of Patients with Human Immunodeficiency Virus Infection -- 96 Quantitation of Viremia and Determination of Drug Resistance in Patients with Human Immunodeficiency Virus Infection -- 97 Measurement of T-Cell-Specific Immunity in Patients with Human Immunodeficiency Virus Infection -- SECTION L: IMMUNODEFICIENCY DISEASES -- 98 The Primary Immunodeficiency Diseases -- 99 Approach to the Diagnosis of Severe Combined Immunodeficiency -- 100 Investigation of Signal Transduction Defects -- 101 Primary Antibody Deficiency Diseases -- 102 Hereditary and Acquired Complement Deficiencies -- 103 Neutropenia and Neutrophil Defects -- SECTION M: ALLERGIC DISEASES -- 104 Introduction -- 105 Quantification and Standardization of Allergens -- 106 In Vivo Diagnostic Allergy Testing -- 107 Immunological Methods in the Diagnostic Allergy Clinical and Research Laboratory -- 108 Assay Methods for Measurement of Mediators and Markers of Allergic Inflammation -- 109 Tests for Immunological Reactions to Foods -- 110 Diagnosis of Hypereosinophilic Syndromes -- SECTION N: SYSTEMIC AUTOIMMUNE DISEASES -- 111 Introduction -- 112 Immunofluorescent Antinuclear Antibody Tests -- 113 Detection of Autoantibodies against Proteins and Ribonucleoproteins by Double Immunodiffusion and Immunoprecipitation -- 114 Detection of Autoantibodies by Using Immobilized Natural and Recombinant Autoantigens -- 115 Detection of Anti-DNA Autoantibodies -- 116 Autoantibody Testing in Rheumatoid Arthritis -- 117 Laboratory Testing for Antibodies Associated with Antiphospholipid Antibody Syndrome -- 118 Antineutrophil Cytoplasm Antibodies (ANCAs) and Strategy for Diagnosing ANCA-Associated Vasculitides.

SECTION O: ORGAN-LOCALIZED AUTOIMMUNE DISEASES -- 119 Introduction -- 120 Endocrinopathies -- 121 Autoantibodies to Glycolipids in Peripheral Neuropathy -- 122 Detection of Antimitochondrial Autoantibodies in Primary Biliary Cirrhosis and Liver Kidney Microsomal Antibodies in Autoimmune Hepatitis -- 123 Skin Diseases -- 124 Cardiovascular Diseases -- 125 Kidney and Lung Disease Mediated by Anti-Glomerular Basement Membrane Antibodies: Detection by Western Blot Analysis -- 126 Autoimmunity of Testis, Ovary, and Spermatozoa -- 127 Immunologic Testing for Celiac Disease and Inflammatory Bowel Disease -- 128 Autoimmune Thrombocytopenia -- 129 Monitoring Autoimmune Reactivity within the Retina -- SECTION P: CANCER -- 130 Introduction -- 131 Immunologic Approaches to Tumor Markers: Assays, Applications, and Discovery -- 132 Malignancies of the Immune System: Use of Immunologic and Molecular Tumor Markers in Classification and Diagnostics -- 133 Monitoring of Immunologic Therapies -- 134 The Future of Cancer Diagnostics: Proteomics, Immunoproteomics, and Beyond -- SECTION Q: TRANSPLANTATION IMMUNOLOGY -- 135 Histocompatibility Testing after Fifty Years of Transplantation -- 136 Molecular Methods: HLA Alleles -- 137 Evaluation of the Humoral Response in Transplantation -- 138 Evaluation of the Cellular Immune Response in Transplantation -- 139 Molecular Characterization of Rejection in Solid Organ Transplantation -- 140 Human Natural Killer Cell Receptors -- 141 Detecting Chimerism after Blood and Marrow Transplantation -- SECTION R: LABORATORY MANAGEMENT -- 142 Clinical Immunology Laboratory Accreditation, Licensure, and Credentials -- Author Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- R -- S -- T -- U -- V -- W -- Y -- Z -- Subject Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O.

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