Antigen Retrieval Immunohistochemistry Based Research and Diagnostics.
Title:
Antigen Retrieval Immunohistochemistry Based Research and Diagnostics.
Author:
Taylor, Clive R.
ISBN:
9780470875605
Personal Author:
Edition:
1st ed.
Physical Description:
1 online resource (470 pages)
Series:
Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems. ; v.5
Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems.
Contents:
ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY BASED RESEARCH AND DIAGNOSTICS -- CONTENTS -- PREFACE -- CONTRIBUTORS -- PART I: RECENT ADVANCES IN ANTIGEN RETRIEVAL TECHNIQUES AND ITS APPLICATION -- CHAPTER 1: STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THE TEST BATTERY APPROACH -- CHAPTER 2: EXTENDED APPLICATION OF ANTIGEN RETRIEVAL TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION -- CHAPTER 3: EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE BASED ON THE ANTIGEN RETRIEVAL PRINCIPLE -- PART II: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY -- CHAPTER 4: KEY ISSUES AND STRATEGIES OF STANDARDIZATION FOR QUANTIFIABLE IMMUNOHISTOCHEMISTRY -- CHAPTER 5: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON ANTIGEN RETRIEVAL TECHNIQUE -- CHAPTER 6: STANDARD REFERENCE MATERIAL: CELL LINE DEVELOPMENT AND USE OF REFERENCE CELL LINES AS STANDARDS FOR EXTERNAL QUALITY ASSURANCE OF HER2 IHC AND ISH TESTING -- CHAPTER 7: PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS -- CHAPTER 8: STANDARD REFERENCE MATERIAL: PROTEIN-EMBEDDING TECHNIQUE AND DESIGN OF BAR CODE -- CHAPTER 9: THE PROS AND CONS OF AUTOMATION FOR IMMUNOHISTOCHEMISTRY FROM THE PROSPECTIVE OF THE PATHOLOGY LABORATORY -- CHAPTER 10: IMAGE ANALYSIS IN IMMUNOHISTOCHEMISTRY -- PART III: TISSUE/CELL SAMPLE PREPARATION -- CHAPTER 11: TISSUE CELL SAMPLE PREPARATION: LESSONS FROM THE ANTIGEN RETRIEVAL TECHNIQUE -- CHAPTER 12: MECHANISMS OF ACTION AND PROPER USE OF COMMON FIXATIVES -- CHAPTER 13: CELL SAMPLE PREPARATION FOR CLINICAL CYTOPATHOLOGY: CURRENT STATUS AND FUTURE DEVELOPMENT -- CHAPTER 14: DESIGN OF A TISSUE SURROGATE TO EXAMINE ACCURACY OF PROTEOMIC ANALYSIS -- PART IV: MOLECULAR MECHANISM OF ANTIGEN RETRIEVAL TECHNIQUE -- CHAPTER 15: STUDY OF FORMALIN FIXATION AND HEAT-INDUCED ANTIGEN RETRIEVAL -- CHAPTER 16: A LINEAR EPITOPES MODEL OF ANTIGEN RETRIEVAL.
CHAPTER 17: pH OR IONIC STRENGTH OF ANTIGEN RETRIEVAL SOLUTION: A POTENTIAL ROLE FOR REFOLDING DURING HEAT TREATMENT -- CHAPTER 18: COMMENTARY: FUTURE DIRECTIONS -- PART V: PROTEOMIC ANALYSIS OF PROTEIN EXTRACTED FROM TISSUE/CELLS -- CHAPTER 19: TECHNIQUES OF PROTEIN EXTRACTION FROM FFPE TISSUE/ CELLS FOR MASS SPECTROMETRY -- CHAPTER 20: APPLICATION OF SHOTGUN PROTEOMICS TO FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUES -- CHAPTER 21: VISUALIZING PROTEIN MAPS IN TISSUE -- CHAPTER 22: SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND PROTEOMICS: MARCHING TO A NEW ERA -- APPENDIX: RELATED LABORATORY PROTOCOLS -- INDEX -- Color plates.
Abstract:
The most complete, up-to-date reference on antigen retrieval and immunohistochemistry An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects. Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work: Summarizes the key problems in the four fields of antigen retrieval Discusses the advances of AR techniques and their applications Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC Includes an appendix of related laboratory protocols Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for
most biotechnology companies majoring in development of IHC products. Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor.
Local Note:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
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Electronic Access:
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