Protein Purification : Principles, High Resolution Methods, and Applications.
Title:
Protein Purification : Principles, High Resolution Methods, and Applications.
Author:
Janson, Jan-Christer.
ISBN:
9780470940075
Personal Author:
Edition:
3rd ed.
Physical Description:
1 online resource (542 pages)
Series:
Methods of Biochemical Analysis ; v.151
Methods of Biochemical Analysis
Contents:
PROTEIN PURIFICATION -- PREFACE TO THE THIRD EDITION -- PREFACE TO THE SECOND EDITION -- PREFACE TO THE FIRST EDITION -- CONTRIBUTORS -- PART I INTRODUCTION -- 1 Introduction to Protein Purification -- PART II CHROMATOGRAPHY -- 2 Introduction to Chromatography -- 3 Gel Filtration: Size Exclusion Chromatography -- 4 Ion Exchange Chromatography -- 5 High-Resolution Reversed-Phase Chromatography of Proteins -- 6 Hydrophobic Interaction Chromatography -- 7 Immobilized Metal Ion Affinity Chromatography -- 8 Covalent Chromatography -- 9 Affinity Chromatography -- 10 Affinity Ligands from Chemical Combinatorial Libraries -- 11 Affinity Ligands from Biological Combinatorial Libraries -- PART III OTHER SEPARATION METHODS AND RELATED TECHNIQUES -- 12 Membrane Separations -- 13 Refolding of Inclusion Body Proteins from E. coli -- 14 Purification of PEGylated Proteins -- PART IV ELECTROPHORESIS -- 15 Electrophoresis in Gels -- 16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients -- 17 Two-Dimensional Electrophoresis in Proteomics -- 18 Protein Elution and Blotting Techniques -- 19 Capillary Electrophoretic Separations -- PART V SEPARATION METHOD OPTIMIZATION -- 20 High Throughput Screening Techniques in Protein Purification -- INDEX.
Abstract:
The authoritative guide on protein purification-now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography.
Local Note:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
Genre:
Electronic Access:
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