CONTENTS -- Introduction: Engineering the Tissue Extracellular Matrix with Hybrid Biomaterials Esmaiel Jabbari -- 1. Introduction -- 2. Synthesis -- 3. Characterization -- 4. Structure -- 5. Applications -- 6. Book Structure -- References -- SECTION 1: Synthesis, Characterization and Self-Assembly -- Chapter 1: Bulk and Solution Properties of Peptide-Polymer Conjugates Autumn Carlsen, Harm-Anton Klok and Sébastien Lecommandoux -- 1. Introduction -- 2. Bulk Self-assembled Structures from Polypeptide-based Conjugates -- 2.1. Diblock copolymer architectures -- Polydiene-based diblock copolymers -- Polystyrene-based diblock copolymers -- Polyether-based diblock copolymers -- Polyester-based diblock copolymers -- Diblock copolypeptides -- Miscellaneous -- 2.2. Triblock copolymer architectures -- Polydiene-based diblock copolymers -- Polystyrene-based triblock copolymers -- Polysiloxane-based triblock copolymers -- Polyether-based triblock copolymers -- Miscellaneous -- Pseudo-phase diagram -- 3. Solution Self-assembled Micellar Structures from Polypeptide-based Conjugates -- 3.1. Polypeptide-based micelles -- Peptide core-forming micelles -- Peptide shell-forming micelles -- Schizophrenic micelles -- 3.2. Polypeptide-based vesicles -- Peptide-polymer conjugate vesicles -- Copolypeptide vesicles -- 3.3. Polypeptide-based hydrogels -- 4. Conclusion and Outlook -- Materials -- Technical analysis -- References -- Chapter 2: Polymer-Peptide Conjugate Networks: Formation, Swelling and Degradation Donald L. Elbert -- 1. Introduction -- 2. Vulcanization -- 2.1. Physical crosslinking -- 2.2. Chemical crosslinking of PEG - commonly used reactions -- 2.3. Chemical crosslinking to produce hybrid PEG materials -- 3. Swelling Properties of PEG Hydrogels -- 4. Predicting Degradation of PEG Hydrogels -- 5. Conclusions -- References.

Chapter 3: Peptide Self-assembly Biomaterials Design and Application Xiaojun Zhao, Songtao Wang, Yangrong Lu and Jingqiu Cheng -- 1. Introduction -- 2. Self-assembling Peptides as Structural Building Motifs -- Peptide lego -- 3. Dynamic Behavior of the Peptide Re-assemblies -- 4. Peptide Scaffolds for Three-dimensional (3D) Cell Cultures -- 5. Peptide Surfactants or Detergents -- 6. Peptide Surfactants Stabilize Membrane Proteins -- Applications of peptide surfactants -- Peptide ink -- 7. Other Peptide Construction Motifs as Material Building Blocks -- 8. Future Trends in Nanobiotechnology -- Acknowledgements -- References -- Chapter 4: Micropatterned Polymer Structures for Cell and Tissue Engineering Kyung-Jin Jang, Deok-Ho Kim, Sun-Min Kim, Andre Levchenko and Kahp-Yang Suh -- 1. Introduction -- 2. Applications of Micropatterned Polymers in Cell Patterning -- 2.1. Microcontact printing -- 2.2. Stencil-based patterning -- 2.3. Microfluidic patterning -- 2.4. Microtopographic patterning -- 3. Microengineering of Cellular Interaction by Polymeric Biomaterials -- 3.1. Patterned co-cultures for controlling cell-cell interactions -- 3.2. Microfabricated 3D polymeric scaffolds for tissue engineering -- 4. Conclusions and Perspective -- Acknowledgements -- References -- Chapter 5: Synthesis and in vitro/in vivo Response to Peptide-Polymer Conjugates Amy S. Chung and Weiyuan John Kao -- 1. Introduction -- 2. Host Response to Biomaterials -- 2.1. Overview: Process and progression -- 2.2. In vitro response to biomaterials -- 2.3. In vivo response to biomaterials -- 2.4. Enhancing biological response: Host interaction with peptide-polymer conjugates -- 3. Peptide-Polymer Conjugate Systems: Synthesis and Interaction with the Host -- 3.1. Overview: Conjugation chemistry and properties -- 3.2. Peptide conjugated natural polymers.

3.2.1. Peptide conjugated natural polymers -- 3.2.2. Case study: Peptide complexed hyaluronic acid -- 3.3. Peptide conjugated synthetic polymers -- 3.3.1. Case study: Poly(ethylene glycol) -- 4. Host Response to Peptide-Polymer Conjugate Systems: Future Directions and Challenges -- References -- SECTION 2: Hybrid Biomaterials in Drug Delivery and Molecular Recognition -- Chapter 6: Synthesis of Multi-epitopic Glycopeptide-based Cancer Vaccines Olivier Renaudet, Isabelle Bossu and Pascal Dumy -- 1. Introduction -- 2. Design of Synthetic Antigen Delivery Systems as Cancer Vaccine Prototypes -- 3. Synthesis of Linear Glycopeptide-based Conjugates and Linkers -- 3.1. Utilization of glycosyl amino acid building blocks -- 3.1.1. Synthesis of mucin antigen-T cell peptide conjugates -- 3.1.2. Synthesis of linear peptide linker bearing clustered carbohydrate antigens -- 3.2. Chemoselective methods -- 3.2.1. Convergent assembly by oxime ligation -- 3.2.2. Native chemical ligation -- 3.2.3. Click-like cycloaddition -- 4. Construction of Multi-epitopic Glycosylated Dendrimeric Type Structures -- 4.1. Multiple Antigen Glycopeptide (MAG) using a polylysine core -- 4.2. Multi-epitopic cyclo-glycopeptide -- 4.3. Other multivalent scaffolds -- 5. Conclusion -- References -- Chapter 7: Stimuli-sensitive Particles for Drug Delivery Stephanie J. Grainger and Mohamed E. H. El-Sayed -- 1. Introduction -- 1.1. pH-sensitive polymers with acidic functional groups -- 1.2. pH-sensitive polymers wth basic functional groups -- 2. Synthesis Strategies for pH-Sensitive Polymers -- 2.1. Reversible addition-fragmentation chain transfer (RAFT) technique -- 2.2. Atom transfer radical polymerization (ATRP) technique -- 2.3. Nitroxide-mediated polymerization (NMP) technique -- 3. "Smart" Particles -- 3.1. Role of "smart" particles in delivery of therapeutic nucleic acids.

3.2. Role of "smart" particles in delivery of peptides and proteins -- 4. Future Directions -- LIST OF ABBREVIATIONS -- References -- Chapter 8: Design and Synthesis of Endosomolytic Conjugated Polyaspartamide for Cytosolic Drug Delivery Kwangwon Seo and Dukjoon Kim -- 1. Introduction -- 2. Synthesis of Poly(aspartamide)s -- 3. Conjugation of Hydrophobic and Hydrophilic Macromers -- 4. Self-aggregation -- 5. Phase Transition Behaviors -- 6. pH-Sensitive Properties -- 7. Hemolytic and Agglutination Properties -- 8. Endosomal Escape and Cytosolic Delivery of Therapeutic Agents -- 9. Biocompatibility -- 10. Summary -- References -- Chapter 9: Engineering of Cell-penetrating Peptide-conjugated Intracellular Delivery Systems Rupa R. Sawant and Vladimir P. Torchilin -- 1. Introduction -- 2. CPP-modified Delivery Systems -- 2.1. Delivery of proteins and peptides -- 2.2. Delivery of nanoparticles -- 2.3. Delivery of nucleic acids -- 2.4. Stimuli-sensitive nanoparticulate drug delivery system -- 3. Problems with CPP-mediated Delivery and Ways to Overcome -- List of Acronyms and Abbreviations -- References -- SECTION 3: Cell Responsive Biomaterials in Tissue Engineering -- Chapter 10: Biomimetic Matrices for Integrin-mediated Cell Adhesion Keshia M. Ashe, Duron A. Lee, Kevin W.-H. Lo, Lakshmi S. Nair and Cato T. Laurencin -- 1. Introduction -- 2. Integrin-mediated Cell Adhesion -- 3. Cellular Adhesion to Implanted Biomaterials -- 4. Biomimetic Materials for Tissue Engineering using ECM Proteins -- 4.1. Physical adsorption of ECM proteins to biomaterials -- 4.1.1. Influencing ECM protein adsorption -- 5. Biomimetic Materials for Tissue Engineering using ECM Peptides -- 5.1. Covalent immobilzation of ECM-derived peptides to biomaterials -- 5.2. Effects of immobilized peptide concentration and distribution -- 6. Bioactive Ceramics for Bone Tissue Engineering.

6.1. Surface coatings -- 6.1.1. Biological apatite -- 6.1.2. Surface bound ECM-derived proteins and peptides -- 7. Conclusion -- References -- Chapter 11: Engineering Artificial Stem Cell Niches Matthias P. Lutolf -- 1. Introduction -- 1.1. Adult stem cells are regulated by niches -- 1.2. Cell-cell interactions in the niche -- 1.3. Stem cell-ECM interactions in the niche -- 1.4. Stem cell interaction with soluble niche signals -- 1.5. Stem cell functions controlled by the niche -- 2. Engineering in vitro Surrogate Models of Stem Cell Niches -- 2.1. Engineering biomaterials with niche-like physicochemical characteristics -- 2.2. Microwell arrays for high-throughput single stem cell studies -- 2.3. Niche protein microarrays -- 2.4. Dissecting cell-cell interactions in the niche in 3D -- 2.5. 3D biomolecule gradients as model niches -- 2.6. Mimicking the spatial 3D niche heterogeneity -- 2.7. From artificial niches to 3D in vitro 'tissues' -- 3. Conclusions and Outlook -- References -- Chapter 12: Engineering Peptides in Hydrogels for Cartilage Tissue Regeneration Zhaoyang Ye and Jennifer Elisseeff -- 1. Introduction -- 2. Hydrogel Systems for Cartilage Tissue Engineering -- 2.1. Synthetic polymer-based hydrogels -- 2.2. Polysaccharide-based hydrogels -- 2.3. Protein-based hydrogels -- 2.4. Self-assembling peptide-based hydrogels -- 3. Cell Sources for Cartilage Tissue Regeneration Chondrocytes -- 3.1. Chondrocytes -- 3.2. Mesenchymal stem cells -- 3.3. Embryonic stem cells -- 4. Rational Selection of Peptides for Cartilage Tissue Engineering -- 4.1. Cell adhesion -- 4.2. Interaction with ECM components -- 4.3. ECM remodeling -- 5. Strategies for Bioconjugation of Peptides with Hydrogels -- 5.1. Pendant attachment of peptides -- 5.2. Crosslinking via peptides -- 5.3. Direct designing self-assembling peptides -- 5.4. Controlled presentation of peptides.

6. Peptide Conjugated Hydrogels for Cartilage Tissue Engineering.