Primary and Stem Cells : Gene Transfer Technologies and Applications.
Title:
Primary and Stem Cells : Gene Transfer Technologies and Applications.
Author:
Lakshmipathy, Uma.
ISBN:
9781118147146
Personal Author:
Edition:
1st ed.
Physical Description:
1 online resource (304 pages)
Contents:
Primary and Stem Cells: Gene Transfer Technologies and Applications -- CONTENTS -- FOREWORD -- PREFACE -- CONTRIBUTORS -- PART I: CLONING AND GENE DELIVERY -- 1. DNA Assembly Technologies Based on Homologous Recombination -- 2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells -- 3. Strategies for the Delivery of Naked DNA -- PART II: NONINTEGRATING TECHNOLOGIES -- 4. Episomal Vectors -- 5. Nonintegrating DNA Virus -- 6. Nonintegrating RNA Viruses -- 7. Protein Delivery -- PART III: INTEGRATING TECHNOLOGIES -- 8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery -- 9. Integrating Viral Vectors for Gene Modifications -- 10. Bacteriophage Integrases for Site-Specific Integration -- 11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells -- PART IV: APPLICATIONS -- 12. Modified Stem Cells as Disease Models and in Toxicology Screening -- 13. Screening and Drug Discovery -- INDEX -- COLOUR PLATES.
Abstract:
This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
Local Note:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
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