Redox Biocatalysis : Fundamentals and Applications.
Title:
Redox Biocatalysis : Fundamentals and Applications.
Author:
Gamenara, Daniela.
ISBN:
9781118409299
Personal Author:
Edition:
1st ed.
Physical Description:
1 online resource (549 pages)
Contents:
REDOX BIOCATALYSIS -- CONTENTS -- PREFACE -- 1. Enzymes Involved in Redox Reactions: Natural Sources and Mechanistic Overview -- 1.1 Motivation: Green Chemistry and Biocatalysis -- 1.2 Sources of Biocatalysts -- 1.2.1 Plants and Animals as Sources of Redox Biocatalysts -- 1.2.2 Wild-Type Microorganisms -- 1.2.2.1 Yeasts -- 1.2.2.2 Fungi -- 1.2.2.3 Bacteria -- 1.2.3 Metagenomic Assessments -- 1.3 Overview of Redox Enzymes -- 1.3.1 Dehydrogenases -- 1.3.1.1 Zn-Dependent Dehydrogenases -- 1.3.1.2 Flavin-Dependent Dehydrogenases -- 1.3.1.3 Pterin-Dependent Dehydrogenases -- 1.3.1.4 Quinoprotein Dehydrogenases -- 1.3.1.5 Dehydrogenases without Prosthetic Group -- 1.3.2 Oxygenases -- 1.3.2.1 Monooxygenases -- 1.3.2.2 Dioxygenases -- 1.3.3 Oxidases -- 1.3.3.1 Iron-Containing Oxidases -- 1.3.3.2 Copper-Containing Oxidases -- 1.3.3.3 Flavin-Dependent Oxidases -- 1.3.4 Peroxidases -- 1.4 Concluding Remarks -- References -- 2. Natural Cofactors and Their Regeneration Strategies -- 2.1 Types of Natural Cofactors-Mechanisms -- 2.2 Cofactor Regeneration -- 2.2.1 Enzymatic Regeneration of Reduced Cofactors -- 2.2.1.1 Substrate-Assisted Method -- 2.2.1.2 Enzyme-Assisted Method -- 2.2.2 Enzymatic Regeneration of Oxidized Cofactors -- 2.2.3 Chemical Regeneration of Cofactors -- 2.2.4 Electrochemical Regeneration of Cofactors -- 2.2.5 Photochemical Regeneration of Cofactors -- 2.3 Concluding Remarks -- References -- 3. Reactions Involving Dehydrogenases -- 3.1 General Considerations -- 3.2 Reduction of Carbonyl Groups -- 3.2.1 Reduction of Aliphatic and Aromatic Ketones -- 3.2.2 Reduction of α- and β-keto Esters and Derivatives -- 3.2.3 Reduction of Diketones -- 3.2.4 Reduction of Aldehydes -- 3.3 Racemization and Deracemization Reactions -- 3.4 Preparation of Amines -- 3.5 Reduction of C-C Double Bonds -- 3.6 Oxidation Reactions.
3.7 Dehydrogenase-Catalyzed Redox Reactions in Natural Products -- 3.8 Concluding Remarks -- References -- 4. Reactions Involving Oxygenases -- 4.1 Monooxygenase-Catalyzed Reactions -- 4.1.1 Hydroxylation of Aliphatic Compounds -- 4.1.2 Hydroxylation of Aromatic Compounds -- 4.1.3 Baeyer-Villiger Reactions -- 4.1.3.1 Classification and Metabolic Role of BVMOs -- 4.1.3.2 Isolated Enzymes versus Whole-Cell Systems (Wild-Type and Recombinant Microorganisms) -- 4.1.3.3 Substrate Profile of Available Baeyer-Villiger Monooxygenases -- 4.1.3.4 Synthetic Applications of BVMOs -- 4.1.4 Epoxidation of Alkenes -- 4.2 Dioxygenase-Catalyzed Reactions -- 4.2.1 Aromatic Dioxygenases -- 4.2.1.1 Dihydroxylation of Aromatic Compounds -- 4.2.1.2 Other Oxidation Reactions Performed by Aromatic Dioxygenases -- 4.2.2 Miscellaneous Dioxygenases -- 4.2.2.1 Lipoxygenase -- 4.3 Concluding Remarks -- References -- 5. Reactions Involving Oxidases and Peroxidases -- 5.1 Oxidase-Catalyzed Reactions -- 5.1.1 Oxidases Acting on C-O Bonds -- 5.1.1.1 Galactose Oxidase -- 5.1.1.2 Pyranose Oxidase -- 5.1.1.3 Alcohol Oxidase -- 5.1.1.4 Glucose Oxidase -- 5.1.1.5 Glycolate Oxidase -- 5.1.2 Laccases and Tyrosinases (Phenol Oxidases) -- 5.1.2.1 Laccase -- 5.1.2.2 Tyrosinase and Other Polyphenol Oxidases -- 5.1.3 Oxidases Acting on C-N Bonds -- 5.1.3.1 D-Amino Acid Oxidase -- 5.1.3.2 L-Amino Acid Oxidase -- 5.1.3.3 Monoamine Oxidase -- 5.1.3.4 Copper Amine Oxidases -- 5.1.4 Miscellaneous -- 5.1.4.1 Cholesterol Oxidase -- 5.1.4.2 Vanillyl Alcohol Oxidase -- 5.1.4.3 Alditol Oxidase -- 5.2 Peroxidase-Catalyzed Reactions -- 5.2.1 Peroxidase Mediated Transformations -- 5.2.1.1 Oxidative Dehydrogenation (2 RH + H2O2 → 2 R· + 2 H2O→R-R) -- 5.2.1.2 Oxidative Halogenation (RH + H2O2 +X- + H+→RX + 2 H2O) -- 5.2.1.3 Oxygen-Transfer Reactions (RH + H2O2 → ROH + H2O) -- 5.3 Concluding Remarks.
References -- 6. Hydrolase-Mediated Oxidations -- 6.1 Hydrolase Promiscuity and in situ Peracid Formation. Perhydrolases vs. Hydrolases. Other Promiscuous Hydrolase-Mediated Oxidations -- 6.2 Hydrolase-Mediated Bulk Oxidations in Aqueous Media (e.g., Bleaching, Disinfection, etc.) -- 6.3 Lipase-Mediated Oxidations: Prileshajev Epoxidations and Baeyer-Villiger Reactions -- 6.4 Hydrolase-Mediated Oxidation and Processing of Lignocellulosic Materials -- 6.5 Concluding Remarks -- References -- 7. Bridging Gaps: From Enzyme Discovery to Bioprocesses -- 7.1 Context -- 7.2 Enzyme Directed Evolution and High-Throughput-Screening of Biocatalysts -- 7.3 Successful Case: Baker's Yeast Redox Enzymes, Their Cloning, and Separate Overexpression -- 7.4 Whole-Cells vs. Isolated Enzymes: Medium Engineering -- 7.5 Beyond: Multistep Domino Biocatalytic Processes -- 7.6 Concluding Remarks -- References -- 8. Industrial Applications of Biocatalytic Redox Reactions: From Academic Curiosities to Robust Processes -- 8.1 Motivation: Drivers for Industrial Biocatalytic Processes -- 8.2 Key Aspects in Industrial Biocatalytic Processes -- 8.3 Industrial Biocatalytic Redox Processes: Free Enzymes -- 8.4 Industrial Biocatalytic Redox Processes-Whole-Cells: The "Designer Bug" Concept and Beyond (Metabolic Engineering) -- 8.5 Concluding Remarks and Future Perspectives -- References -- INDEX.
Abstract:
Paves the way for new industrial applications using redox biocatalysis Increasingly, researchers rely on the use of enzymes to perform redox processes as they search for novel industrial synthetic routes. In order to support and advance their investigations, this book provides a comprehensive and current overview of the use of redox enzymes and enzyme-mediated oxidative processes, with an emphasis on the role of redox enzymes in chemical transformations. The authors examine the full range of topics in the field, from basic principles to new and emerging research and applications. Moreover, they explore everything from laboratory-scale procedures to industrial manufacturing. Redox Biocatalysis begins with a discussion of the biochemical features of redox enzymes as well as cofactors and cofactor regeneration methods. Next, the authors present a variety of topics and materials to the research and development of full-scale industrial applications, including: Biocatalytic applications of redox enzymes such as dehydrogenases, oxygenases, oxidases, and peroxidases Enzyme-mediated oxidative processes based on biocatalytic promiscuity All the steps from enzyme discovery to robust industrial processes, including directed evolution, high-throughput screening, and medium engineering Case studies tracing the development of industrial applications using biocatalytic redox reactions Each chapter ends with concluding remarks, underscoring the key scientific principles and processes. Extensive references serve as a gateway to the growing body of research in the field. Researchers in both academia and industry will find this book an indispensable reference for redox biotransformations, guiding them from underlying core principles to new discoveries and emerging industrial applications.
Local Note:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
Genre:
Electronic Access:
Click to View