Front Cover -- Liquid Chromatography: Fundamentals and Instrumentation -- Copyright -- Contents -- Contributors -- Chapter 1 - Milestones in the Development of Liquid Chromatography -- 1.1. INTRODUCTION -- 1.2. HPLC THEORY AND PRACTICE -- 1.3. COLUMNS -- 1.4. EQUIPMENT -- 1.5. DETECTORS -- APOLOGIES AND ACKNOWLEDGEMENTS -- References -- Chapter 2 - Kinetic Theories of Liquid Chromatography -- 2.1. INTRODUCTION -- 2.2. MACROSCOPIC KINETIC THEORIES -- 2.3. MICROSCOPIC KINETIC THEORIES -- 2.4. COMPARISON OF THE MICROSCOPIC AND MACROSCOPIC MODELS -- References -- Chapter 3 - Column Technology in Liquid Chromatography -- 3.1. INTRODUCTION -- 3.2. COLUMN DESIGN AND HARDWARE -- 3.3. COLUMN PACKING MATERIALS AND STATIONARY PHASES -- 3.4. COLUMN SYSTEMS AND OPERATIONS -- 3.5. CHROMATOGRAPHIC COLUMN TESTING AND EVALUATION -- 3.6. COLUMN MAINTENANCE AND TROUBLESHOOTING -- 3.7. TODAY'S COLUMN MARKET-AN EVALUATION, COMPARISON, AND CRITICAL APPRAISAL -- References -- Chapter 4 - Secondary Chemical Equilibria in Reversed-Phase Liquid Chromatography -- 4.1. INTRODUCTION -- 4.2. ACID-BASE EQUILIBRIA -- 4.3. ION-INTERACTION CHROMATOGRAPHY -- 4.4. MICELLAR LIQUID CHROMATOGRAPHY -- 4.5. METAL COMPLEXATION -- Further Reading -- Chapter 5 - Hydrophilic Interaction Liquid Chromatography -- 5.1. INTRODUCTION -- 5.2. PRINCIPLES OF HILIC -- 5.3. MOBILE AND STATIONARY PHASES COMMONLY EMPLOYED IN HILIC -- 5.4. APPLICATION EXAMPLES -- References -- Chapter 6 - Hydrophobic Interaction Chromatography -- 6.1. INTRODUCTION -- 6.2. PRINCIPLES OF HYDROPHOBIC INTERACTION CHROMATOGRAPHY -- 6.3. MAIN FACTORS THAT AFFECT HYDROPHOBIC INTERACTION CHROMATOGRAPHY -- 6.4. PURIFICATION STRATEGIES -- 6.5. PRACTICAL ASPECTS OF HYDROPHOBIC INTERACTION CHROMATOGRAPHY PURIFICATION -- 6.6. SELECTED APPLICATIONS -- 6.7. FUTURE TRENDS -- References -- Chapter 7 - Liquid-Solid Chromatography.

7.1. INTRODUCTION -- 7.2. RETENTION AND SEPARATION -- 7.3. METHOD DEVELOPMENT -- 7.4. PROBLEMS IN THE USE OF NORMAL-PHASE CHROMATOGRAPHY -- References -- Chapter 8 - Ion Chromatography -- 8.1. INTRODUCTION -- 8.2. BASIC PRINCIPLES AND SEPARATION MODES -- 8.3. INSTRUMENTATION -- 8.4. APPLICATIONS -- References -- Chapter 9 - Size-Exclusion Chromatography -- 9.1. INTRODUCTION -- 9.2. HISTORICAL BACKGROUND -- 9.3. RETENTION IN SIZE-EXCLUSION CHROMATOGRAPHY -- 9.4. BAND BROADENING IN SIZE-EXCLUSION CHROMATOGRAPHY -- 9.5. RESOLUTION IN SIZE-EXCLUSION CHROMATOGRAPHY -- 9.6. SIZE-EXCLUSION CHROMATOGRAPHY ENTERS THE MODERN ERA: THE DETERMINATION OF ABSOLUTE MOLAR MASS -- 9.7. SIZE-EXCLUSION CHROMATOGRAPHY TODAY: MULTIDETECTOR MEASUREMENTS, PHYSICOCHEMICAL CHARACTERIZATION, TWO-DIMENSIONAL TECHNIQUES -- 9.8. CONCLUSIONS -- ACKNOWLEDGEMENT AND DISCLAIMER -- References -- Chapter 10 - Solvent Selection in Liquid Chromatography -- 10.1. ELUTION STRENGTH -- 10.2. COLUMNS AND SOLVENTS IN RPLC, NPLC, AND HILIC -- 10.3. ASSESSMENT OF THE ELUTION STRENGTH -- 10.4. SCHOENMAKERS'S RULE -- 10.5. ISOELUOTROPIC MIXTURES -- 10.6. SOLVENT-SELECTIVITY TRIANGLES -- 10.7. PRACTICAL GUIDELINES FOR OPTIMIZATION OF MOBILE PHASE COMPOSITION -- 10.8. ADDITIONAL CONSIDERATIONS FOR SOLVENT SELECTION -- References -- Chapter 11 - Method Development in Liquid Chromatography -- 11.1. INTRODUCTION -- 11.2. GOALS -- 11.3. A STRUCTURED APPROACH TO METHOD DEVELOPMENT -- 11.4. METHOD DEVELOPMENT IN PRACTICE -- 11.5. PREVALIDATION -- 11.6. VALIDATION -- 11.7. DOCUMENTATION -- 11.8. SUMMARY -- References -- Chapter 12 - Theory and Practice of Gradient Elution Liquid Chromatography -- 12.1. INTRODUCTION -- 12.2. THE EFFECTS OF EXPERIMENTAL CONDITIONS ON SEPARATION -- 12.3. METHOD DEVELOPMENT -- 12.4. PROBLEMS ASSOCIATED WITH GRADIENT ELUTION -- References -- Chapter 13 - General Instrumentation.

13.1. INTRODUCTION -- 13.2. SOLVENT SOURCE -- 13.3. PUMPING SYSTEMS -- 13.4. GRADIENT-ELUTION MIXING SYSTEMS -- 13.5. SAMPLE INJECTION -- 13.6. COLUMN COMPARTMENT -- 13.7. TUBINGS AND FITTINGS -- 13.8. DETECTOR OVERVIEW -- 13.9. ULTRAVIOLET-VISIBLE ABSORBANCE DETECTORS -- 13.10. REFRACTIVE INDEX DETECTORS -- 13.11. EVAPORATIVE LIGHT-SCATTERING DETECTORS -- 13.12. CHARGED AEROSOL DETECTORS -- 13.13. CONDUCTIVITY DETECTORS -- 13.14. FLUORESCENCE DETECTORS -- 13.15. ELECTROCHEMICAL DETECTORS -- 13.16. OTHER DETECTION METHODS -- References -- Chapter 14 - Advanced Spectroscopic Detectors for Identification and Quantification: Mass Spectrometry -- 14.1. INTRODUCTION -- 14.2. IONIZATION METHODS SUITABLE FOR LC COUPLING -- 14.3. HOW TO INCREASE SPECIFICITY OF MS DATA -- 14.4. MICRO- AND NANO-LC-MS -- 14.5. CAPILLARY ELECTROCHROMATOGRAPHY -- References -- Chapter 15 - Advanced Spectroscopic Detectors for Identification and Quantification: FTIR and Raman -- 15.1. INTRODUCTION -- 15.2. OFF-LINE HYPHENATION -- 15.3. ON-LINE HYPHENATION -- 15.4. CONCLUSIONS -- References -- Chapter 16 - Advanced Spectroscopic Detectors for Identification and Quantification: Nuclear Magnetic Resonance -- 16.1. INTRODUCTION -- 16.2. HYPHENATION OF NMR WITH HPLC -- 16.3. ADVANCES IN NMR SENSITIVITY -- 16.4. STRATEGIES FOR OBTAINING NMR INFORMATION FROM A GIVEN LC PEAK -- 16.5. INTEGRATION WITH A MULTIPLE DETECTION SYSTEM (LC-NMR-MS) -- 16.6. QUANTIFICATION CAPABILITIES -- 16.7. FIELDS OF APPLICATION -- 16.8. CONCLUSIONS -- Acknowledgments -- References -- Chapter 17 - Quantitative Structure Property (Retention) Relationships in Liquid Chromatography -- 17.1. INTRODUCTION -- 17.2. METHODOLOGY AND GOALS OF QSRR STUDIES -- 17.3. APPLICATIONS OF QSRR IN PROTEOMICS -- 17.4. CHARACTERIZATION OF STATIONARY PHASES -- 17.5. QSRR AND ASSESSMENT OF LIPOPHILICITY OF XENOBIOTICS.

17.6. QSRR ANALYSIS OF RETENTION DATA DETERMINED ON IMMOBILIZED-BIOMACROMOLECULE STATIONARY PHASES -- 17.7. QUANTITATIVE RETENTION-(BIOLOGICAL) ACTIVITY RELATIONSHIPS -- 17.8. CHEMOMETRICALLY PROCESSED MULTIVARIATE CHROMATOGRAPHIC DATA IN RELATION TO PHARMACOLOGICAL PROPERTIES OF DRUGS AND "DRUG CA ... -- 17.9. CONCLUDING REMARKS -- Acknowledgment -- References -- Chapter 18 - Modeling of Preparative Liquid Chromatography -- 18.1. INTRODUCTION -- 18.2. COLUMN MODEL -- 18.3. ADSORPTION MODEL -- 18.4. PROCESS OPTIMIZATION OF PREPARATIVE CHROMATOGRAPHY -- 18.5. CASE EXAMPLE -- References -- Chapter 19 - Process Concepts in Preparative Chromatography -- 19.1. INTRODUCTION -- 19.2. CLASSICAL ISOCRATIC DISCONTINUOUS ELUTION CHROMATOGRAPHY -- 19.3. OTHER DISCONTINUOUS OPERATING CONCEPTS -- 19.4. CONTINUOUS CONCEPTS OF PREPARATIVE CHROMATOGRAPHY -- 19.5. OPTIMIZATION AND CONCEPT COMPARISON -- 19.6. CONCLUSIONS -- Acknowledgments -- References -- Chapter 20 - Miniaturization and Microfluidics -- 20.1. INTRODUCTION, DEFINITIONS, AND SCOPE -- 20.2. ICROFLUIDIC SYSTEMS FOR SEPARATIONS -- 20.3. COMMERCIAL INSTRUMENTATION -- 20.4. CONCLUSION -- Acknowledgment -- References -- Chapter 21 - Capillary Electrochromatography: A Look at Its Features and Potential in Separation Science -- 21.1. INTRODUCTION -- 21.2. PRINCIPLES OF CAPILLARY ELECTROCHROMATOGRAPHY -- 21.3. INSTRUMENTATION -- 21.4. METHOD OPTIMIZATION IN CEC -- 21.5. EXAMPLES OF SOME RECENT APPLICATIONS -- 21.6. CONCLUSIONS AND FUTURE TRENDS -- References -- Index.