Cover -- Preface -- Acknowledgements -- Contents -- Chapter 1 Introduction -- 1.1 Definition to Tissue Culture -- 1.2 Shoot-Tip and Meristem-Tip Culture -- 1.3 Important Contributors to Plant Tissue Culture -- Chapter 2 Laboratory Organization -- 2.1 Tissue Culture Laboratory Setup -- 2.2 Essential Equipments -- 2.3 Control of Growing Conditions -- 2.4 Green House Facility -- Chapter 3 Methods of Sterilization -- 3.1 Contaminants -- 3.2 Laminar Air Flow (LAF) -- 3.3 Sterilization of Glass Ware -- 3.4 Wet Heat Sterilization: Autoclave -- 3.5 Sterilization of Media and Plant Tissues -- 3.6 Preparation of Stock Plant and Stock Solution -- Chapter 4 Methods of Media Preparation -- 4.1 Medium Stock Solutions -- 4.2 Preparation of Molar Solution -- Chapter 5 Ingredients of Culture Media and Their Functions -- 5.1 Media Components -- 5.2 Media Formulations -- 5.3 Plant Growth Regulators -- 5.4 Light and the Growth Response -- Chapter 6 Plant Hormones -- 6.1 Plant Hormones -- 6.2 Auxins -- 6.3 Cytokinins -- 6.4 Gibberellins -- 6.5 Abscisic Acid -- 6.6 Ethylene -- 6.7 Other Potential PGRS -- Chapter 7 Micro Propagation -- 7.1 Shoot Meristems -- 7.2 Advantages of Shoot Culture -- 7.3 Disadvantages of Shoot Culture -- Chapter 8 Somatic Embryogenesis -- 8.1 Totipotency of the Plant Cell -- 8.2 Uses of Somatic Embryogenesis -- Chapter 9 Synthetic Seeds -- 9.1 Introduction -- 9.2 General Method and Uses -- 9.3 Preparation of Synthetic Seeds -- Chapter 10 Bioreactors as a Low Cost Option for Tissue Culture -- 10.1 Bioreactor Vessels -- 10.2 Types of Bioreactors -- 10.3 Advantages and Disadvantages -- 10.4 Construction of a Simple Bioreactor -- Chapter 11 Disease Detection and Elimination, Quality Assurance -- 11.1 Various Contaminations and Infections -- 11.2 Elisa Test -- 11.3 Polymerase Chain Reaction -- 11.4 Nucleic Acid Hybridization Test.

11.5 Elimination of Viruses -- 11.6 Quality Assurance -- 11.7 DNA Based Molecular Markers -- Chapter 12 Applications of Biotechnology in Horticulture -- 12.1 Tissue Culture -- 12.2 Genetic Engineering of Plants -- 12.3 Engineering Pathogen Resistance -- 12.4 Fruit Quality -- 12.5 Male Sterility and Fertility Restoration -- 12.6 Molecular Diagnostics -- 12.7 Molecular Markers -- 12.8 Development of Beneficial Microbes -- Chapter 13 Genetic Transformation of Plants By Agrobacterium Tumefaciens -- 13.1 The Mechanism of T-DNA Transfer From Agrobacterium to the Plant Cell -- 13.2 Agrobacterium As A Biotechnological Tool -- 13.3 Monocots Transformation -- 13.4 Culture Media -- Chapter 14 Polythylene Glycol (PEG) Mediated Direct Gene Transfer to Plants -- 14.1 Procedure and Solutions -- 14.2 Isolation of Mesophyll Protoplasts From Tobacco Shoots Cultures -- 14.3 Direct Gene Transfer to Protoplasts Using Polyethylene Glycol -- 14.4 Culture of Protoplasts -- 14.5 Isolation of Genomic DNA From Plant Tissue -- Chapter 15 Status of Cryopreservation Technologies in Plants (Crops and Forest Trees) -- 15.1 Theoretical Basis of Plant Cryopreservation -- 15.2 Available Plant Cryopreservation Protocols -- 15.3 Application of Cryopreservation to Herbaceous Species -- 15.4 Application of Cryoplreservation to Woody Species -- Chapter 16 Applications of Cell and Tissue Culture -- 16.1 Clonal Propagation and Micropropagation -- 16.2 Somaclonal Variation -- 16.3 Production of Virus Free Plants -- 16.4 Production of Synthetic Seeds -- 16.5 Production of Secondary Metabolites -- 16.6 Somatic Hybridization -- 16.7 In Vitro Plant Germplasm Conservation -- 16.8 Transgenic Plants As Bioreactors (Molecular Farming) -- 16.9 Edible Vaccines -- 16.10 Molecular Markers -- Chapter 17 Laboratory Protocols -- 17.1 Callus Induction -- 17.2 Suspension Culture -- 17.3 Micropropagation.

17.4 Somatic Embryogenesis -- 17.5 Haploid Production -- 17.6 Protoplast Isolation -- 17.7 Production of Secondary Metabolites -- 17.8 Rapd of Genomic DNA From Plants -- 17.9 Microsatellite Market Analysis of Plant Genomic DNA -- Appendix-A -- Appendix-B -- Appendix-C -- References -- Glossary -- Index.