INDUCED STEM CELLS -- INDUCED STEM CELLS -- Library of Congress Cataloging-in-Publication Data -- Contents -- Preface -- Chapter I: Cryopreservation of Pluripotent Stem Cells: From Slow Cooling Protocols to Slow Cooling Protocols -- Abstract -- Introduction -- 1. Poor Results of Conventional Slow Cooling Protocols -- 2. And Yet We Continue to Use Slow Cooling Protocols -- 3. Why are Human ES Cells Vulnerable to Conventional Slow Cooling Protocols? -- A. How do mES Cells and hES Cells Differ? -- B. What Happens in Cellular Clumps During Conventional Slow Cooling Protocols? -- C. Main Intercellular Junctions of hES Cells: E-Cadherins -- 3. Does the Survival Rate of hES CellsAfter Thawing Increase ifSupercooling is Regulated -- 4. What is Vitrification? -- A. Is Vitrification Effective in hES Cell Clump Freezing? -- B. What are the Disadvantages of Vitrification? -- 5. Back to Slow Cooling Protocols -- A. Ringleader of the Breakthrough-The Rock Inhibitor -- B. What is the Mechanism of Cell Death Occurring inCryopreserved Single hES Cells and its Suppression by theRock Inhibitor? -- 6. What Cryopreservation Methodsare Used for hiPS Cells? -- Conclusion -- Acknowledgments -- References -- Chapter II: Bone Tissue Engineering Approaches and Challenges Using Bioactive Ceramic Scaffolds -- Abstract -- 1. Introduction -- 2. Bone Tissue Engineering -- 3. Bioactive Ceramics -- 4. Tissue Engineering Approacheswith Bioactive Ceramics -- 4.1. Tissue in-Growth -- 4.2. Cell Transplantation -- 4.3. Signaling Molecules -- Conclusion -- Acknowledgments -- References -- Chapter III: Induced Pluripotent Stem Cell-Derived Hepatocytes as an Alternative to Human Adult Hepatocytes -- Abstract -- 1. Introduction -- 2. Generation of Pluripotent Stem Cellsfrom Somatic Cells -- 3. Overview of Generation ofHepatocytes from ES Cells -- 3.1. Development of the Liver.

3.2. Strategy for Commitment of ES Cells into Hepatocytes -- 4. Generation of Hepatocytesfrom iPS Cells -- 4.1. Current Status of Research onHepatic Differentiation of iPS Cells -- 4.2. Possibility of iPS Cells as a Cell Source for CellReplacement Therapy -- 4.3. Possibility of iPS Cell-Derived Hepatocytes as a CellSource for Drug Screening and In Vitro Hepatic Disease Model -- 4.4. Comparison among ES Cell-Derived Hepatocytes, iPSCell-Derived Hepatocytes, and Primary Hepatocytes -- 5. Challenging Issues in Applicationof iPS Cell-Derived Hepatocytes -- 5.1. Cell Replacement Therapy -- 5.2. Drug Screening -- 5.3. Disease Model -- 6. Possibility of Direct Conversion ofSomatic Differentiated or Stem Cells intoFunctional Hepatocytes -- Conclusion -- References -- Chapter IV: Production of Cells with the Patient's Genotype by Induced Pluripotent Stem Cell Technique -- Preface -- Induced Pluripotent Stem Cells (iPSCs) -- Other Methods to AchieveReprogramming - 1.Cloning = SomaticCell Nuclear Transfer (SCNT) 2.Cell Fusion3.Egg Extract -- Mechanisms of Reprogramming -- History of Reprogramming -- Transplanting Cells -- Direct Programming of SomaticCells Into Another Cell Type -- Final Remarks -- References -- Chapter V: Induced Pluripotent Stem Cell Technology for the Study of Neurodegenerative Diseases -- Abstract -- Introduction -- Main Document -- Conclusion -- Acknowledgments -- Sources of Funding -- References -- Abstract -- Chapter VI: Generation of Human Induced Pluripotent Stem Cells from Cord Blood Cells -- Introduction -- 1. Reprogramming of CD34+Cells from Cord Blood. -- 2. Establishment of iPS Cellsfrom CD34+ CB Cells -- Section 2.1. Immunohistological Staining of ES Cell-LikeClones -- Section 2.2. Karyotype and Gene Chip Analysis of an ES Cell-Like Clone -- Section 2.3 Differentiation Potential of ES Cell-Like Clones InVitro and In Vivo.

3. Maintenance of CBCell-Derived iPS Cells -- Section 3.1. Maintenance of ips Cells On Feeder Cells -- Section 3.2. Maintenance of ips Cells without Feeder Cells -- Acknowledgments -- References -- Index.