1 Genetic Manipulation of Mammalian Cells -- 1.1 Hererologous Expression of Genes in Mammalian Cells -- 1.2 Permanent Gene Expression in Mammalian Cells: Gene Transfer and Selection -- 1.3 Vectors for Gene Transfer and Expression in Animal Cells -- 1.4 Aspects of Gene Transfer and Gene Amplification in Recombinant Mammalian Cells -- 1.5 Isolation of Recombinant Cell Clones Exhibiting High-Level Expression of the Introduced Gene -- 1.6 Genetic Engineering of Antibodies and Derivatives from Mammalian Cells -- 1.7 Safety Evaluation of Products Derived From Mammalian Cell Lines -- 2 Biological Aspects of Animal Cells -- 2.1 Metabolic Control of Animal Cell Culture Processes -- 2.2 Glycosylation: A Post-Translational Modification -- 3 Cell Cultivation Technology -- 3.1 Bioreactors Designed for Animal Cells -- 3.2 Hydrodynamic Properties in Bioreactors -- 3.3 Kinetics and Simulation of Animal Cell Processes -- 3.4 On-line and Off-line Process Analysis -- 4 Down-Stream Processing -- 4.1 Principles of Product Extraction from Cell Culture and Purification for Pharmaceutical Proteins -- 4.2 Validation of Downstream Processes -- Index.