In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
Title:
In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
Author:
Scandola, Cyril
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (495 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to analyze ultrastructure of the megakaryocytes in situ using transmission electron microscopy (TEM). Murine bone marrows are collected, fixed, embedded in epoxy resin and cut in ultrathin sections. After contrast staining, the bone marrow is observed under a TEM microscope at 120 kV.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62494