Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
Title:
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
Author:
Wibisono, Phillip
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (369 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here, we describe a detailed protocol for isolating active nuclear extract from larval stage 4 C. elegans and visualizing transcription activity in an in vitro system.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62723