Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
Title:
Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
Author:
Franke, Tilman
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (587 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We describe the preparation of ribbons of serial sections and their collection on large transfer support for use as Array Tomography samples, along with automated imaging procedures in a scanning electron microscope. The protocol allows screening, retrieval, and targeted imaging of local, rare events, and the acquisition of large data volumes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61847