A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
Title:
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
Author:
Kobayashi, Hiroshi
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (434 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61938