Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
Title:
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
Author:
Poulton, Beth Crawford
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (389 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62131