Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
Title:
Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
Author:
Courson, Justin A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (561 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This protocol outlines a routine method for using serial block-face scanning electron microscopy (SBF-SEM), a powerful 3D imaging technique. Successful application of SBF-SEM hinges on proper fixation and tissue staining techniques, as well as careful consideration of imaging settings. This protocol contains practical considerations for the entirety of this process.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62045