Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
Title:
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
Author:
See, Ji-Eun
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (562 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61557