
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Title:
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Author:
LaRiviere, Wells B.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (357 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This report describes techniques to isolate and purify sulfated glycosaminoglycans (GAGs) from biological samples and a polyacrylamide gel electrophoresis approach to approximate their size. GAGs contribute to tissue structure and influence signaling processes via electrostatic interaction with proteins. GAG polymer length contributes to their binding affinity for cognate ligands.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62319