Assays for Validating Histone Acetyltransferase Inhibitors
Title:
Assays for Validating Histone Acetyltransferase Inhibitors
Author:
Waddell, Aaron R.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (551 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Inhibitors of histone acetyltransferases (HATs, also known as lysine acetyltransferases), such as CBP/p300, are potential therapeutics for treating cancer. However, rigorous methods for validating these inhibitors are needed. Three in vitro methods for validation include HAT assays with recombinant acetyltransferases, immunoblotting for histone acetylation in cell culture, and ChIP-qPCR.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61289