TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Title:
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Author:
Zhang, Yongliang
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (422 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60728