Production and Characterization of Human Macrophages from Pluripotent Stem Cells
Title:
Production and Characterization of Human Macrophages from Pluripotent Stem Cells
Author:
Lopez-Yrigoyen, Martha
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (485 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
This protocol describes the robust generation of macrophages from human induced pluripotent stem cells, and methods for their subsequent characterization. Cell surface marker expression, gene expression, and functional assays are used to assess the phenotype and function of these iPSC-derived macrophages.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61038