Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
Title:
Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
Author:
Stolwijk, Judith A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (373 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60686