Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
Title:
Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
Author:
Zhang, Yan-Ru
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (609 seconds)
Series:
Environment
General Note:
Title from resource description page
Abstract:
In order to observe ultrastructure of insect sensilla, scanning and transmission electron microscopy (SEM and TEM, respectively) sample preparation protocol were presented in the study. Tween 20 was added into the fixative to avoid sample deformation in SEM. Fluorescence microscopy was helpful for improving slicing accuracy in TEM.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59251