Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment
Title:
Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment
Author:
Hsiao, Chi-Hao
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (671 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3+CD56+ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60420