Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
Title:
Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
Author:
Hamilton, Alexander
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (483 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is performed.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59491