Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
Title:
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
Author:
Herb, Marc
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (406 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Macrophages, especially primary macrophages, are challenging to transfect as they specialize in detecting molecules of non-self origin. We describe a protocol that allows highly efficient transfection of primary macrophages with mRNA generated from DNA templates such as plasmids.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60143